Clonal Dissemination of NDM-Producing Proteus mirabilis in a Teaching Hospital in Sousse, Tunisia.

IF 3.3 3区医学 Q2 MICROBIOLOGY

Pathogens Pub Date : 2025-03-20 DOI:10.3390/pathogens14030298

Nadia Jaidane, Lamia Tilouche, Saoussen Oueslati, Delphine Girlich, Sana Azaiez, Aymeric Jacquemin, Laurent Dortet, Walid Naija, Abdelhalim Trabelsi, Thierry Naas, Wejdene Mansour, Rémy A Bonnin

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引用次数: 0

Abstract

Proteus mirabilis (P. mirabilis) is an opportunistic pathogen involved in urinary tract infections as well as various nosocomial infections. Emerging resistances to beta-lactams in this species complicates potential treatment since it is intrinsically resistant to colistin. Eleven isolates of carbapenem-non-susceptible P. mirabilis were identified in Sousse Hospital, Tunisia, from January 2018 to December 2022. MICs were determined and isolates were sequenced to determine their resistomes, sequence types, virulence factors, and their clonal relationships. Susceptibility testing showed that all isolates were resistant to carbapenems, aminoglycosides, fluoroquinolones, chloramphenicol, and the trimethoprim/sulfamethoxazole combination. They remained susceptible to the aztreonam/avibactam combination. All isolates produced NDM-1 carbapenemase and ArmA 16S rRNA methylase. In addition, one isolate co-produced the bla_VEB-6 gene. All isolates belonged to ST135, and phylogenetic analysis revealed that they were closely related. This study described the first outbreak of NDM-1-producing P. mirabilis in Tunisia.

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mirabilis 变形杆菌（P. mirabilis）是一种机会性病原体，可引起尿路感染和各种院内感染。由于该菌对可乐定具有固有耐药性，因此该菌对β-内酰胺类药物的新耐药性使潜在的治疗变得更加复杂。2018 年 1 月至 2022 年 12 月期间，在突尼斯苏塞医院发现了 11 个碳青霉烯类不耐药的 mirabilis P. 分离株。确定了 MICs，并对分离株进行了测序，以确定其抗药性组、序列类型、毒力因子及其克隆关系。药敏试验显示，所有分离株都对碳青霉烯类、氨基糖苷类、氟喹诺酮类、氯霉素和三甲双胍/磺胺甲噁唑复方制剂耐药。它们对阿曲南胺/阿维菌素复方制剂仍然敏感。所有分离物都产生 NDM-1 碳青霉烯酶和 ArmA 16S rRNA 甲基化酶。此外，一个分离株还同时产生 blaVEB-6 基因。所有分离株都属于 ST135，系统发育分析表明它们之间有密切的亲缘关系。本研究描述了突尼斯首次爆发的产NDM-1的奇异变形杆菌。

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来源期刊

Pathogens Medicine-Immunology and Allergy

CiteScore

6.40

自引率

8.10%

发文量

1285

审稿时长

17.75 days

期刊介绍： Pathogens (ISSN 2076-0817) publishes reviews, regular research papers and short notes on all aspects of pathogens and pathogen-host interactions. There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.