The Composition of the Dispersion Medium Determines the Antibacterial Properties of Copper (II) Oxide Nanoparticles Against Escherichia coli Bacteria.

IF 4.4 3区材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Nanomaterials Pub Date : 2025-03-20 DOI:10.3390/nano15060469

Olga V Zakharova, Alexander A Gusev, Peter A Baranchikov, Svetlana P Chebotaryova, Svetlana S Razlivalova, Elina Y Koiava, Anna A Kataranova, Gregory V Grigoriev, Nataliya S Strekalova, Konstantin V Krutovsky

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Abstract

Copper (II) oxide nanoparticles (CuO NPs) attract much attention as a promising antimicrobial agent. We studied the antibacterial properties of three types of CuO NPs against Escherichia coli bacteria: flake-shaped particles with a diameter of 50-200 nm and a thickness of 10-20 nm (CuO-CD synthesized by chemical deposition), spherical particles with a size of 20-90 nm (CuO-EE obtained by electrical explosion), and rod-shaped particles with a length of 100-200 nm and a diameter of 30 × 70 nm (CuO-CS commercial sample). We tested how the shape, size, and concentration of the NPs, and composition of the dispersion medium affected the properties of the CuO NPs. We prepared dispersions based on distilled water, a 0.9% NaCl solution, and the LB broth by Lennox and used Triton X-100 and sodium dodecyl sulfate (SDS) as stabilizers. The concentration of NPs was 1-100 mg L^-1. We showed that the dispersion medium composition and stabilizer type had the greatest influence on the antibacterial effects of CuO NPs. We observed the maximum antibacterial effect for all CuO NP types dispersed in water without a stabilizer, as well as in LB broth with the SDS stabilizer. The maximum inhibition of culture growth was observed under the influence of CuO-EE (by 30%) and in the LB broth with the SDS stabilizer (by 1.3-1.8 times depending on the type of particles). In the saline solution, the antibacterial effects were minimal; in some cases, the CuO NPs even promoted bacterial culture growth. SDS increased the antibacterial effects of NPs in broth and saline but decreased them in water. Finally, among the particle types, CuO-CS turned out to be the most bactericidal, which is probably due to their rod-shaped morphology and small diameter. At the same time, the concentration and aggregation effects of CuO NPs in the colloidal systems we studied did not have a linear action on their antibacterial properties. These results can be used in the development of antibacterial coatings and preparations based on CuO NPs to achieve their maximum efficiency, taking into account the expected conditions of their use.

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氧化铜（II）纳米粒子（CuO NPs）作为一种前景广阔的抗菌剂备受关注。我们研究了三种 CuO NPs 对大肠杆菌的抗菌特性：直径为 50-200 nm、厚度为 10-20 nm 的片状颗粒（通过化学沉积合成的 CuO-CD），尺寸为 20-90 nm 的球状颗粒（通过电爆炸获得的 CuO-EE），以及长度为 100-200 nm、直径为 30 × 70 nm 的杆状颗粒（CuO-CS 商业样品）。我们测试了 NPs 的形状、大小和浓度以及分散介质的成分对 CuO NPs 性能的影响。我们以蒸馏水、0.9% NaCl 溶液和 Lennox 的 LB 肉汤为基础制备分散液，并使用 Triton X-100 和十二烷基硫酸钠（SDS）作为稳定剂。NPs 的浓度为 1-100 mg L-1。结果表明，分散介质成分和稳定剂类型对 CuO NPs 的抗菌效果影响最大。我们观察到，所有类型的 CuO NP 在不含稳定剂的水中和含 SDS 稳定剂的 LB 肉汤中的抗菌效果都最大。在 CuO-EE 的影响下（30%），以及在含有 SDS 稳定剂的 LB 肉汤中（1.3-1.8 倍，视颗粒类型而定），培养物的生长受到了最大程度的抑制。在生理盐水中，抗菌效果微乎其微；在某些情况下，CuO NPs 甚至会促进细菌培养物的生长。SDS 增加了肉汤和生理盐水中 NPs 的抗菌效果，但降低了水中的抗菌效果。最后，在各种类型的粒子中，CuO-CS 的杀菌效果最好，这可能是由于其杆状形态和较小的直径。同时，在我们研究的胶体系统中，CuO NPs 的浓度和聚集效应并没有对其抗菌特性产生线性影响。这些结果可用于开发基于 CuO NPs 的抗菌涂层和制剂，以便在考虑到其预期使用条件的情况下实现其最大效率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nanomaterials NANOSCIENCE & NANOTECHNOLOGY-MATERIALS SCIENCE, MULTIDISCIPLINARY

CiteScore

8.50

自引率

9.40%

发文量

3841

审稿时长

14.22 days

期刊介绍： Nanomaterials (ISSN 2076-4991) is an international and interdisciplinary scholarly open access journal. It publishes reviews, regular research papers, communications, and short notes that are relevant to any field of study that involves nanomaterials, with respect to their science and application. Thus, theoretical and experimental articles will be accepted, along with articles that deal with the synthesis and use of nanomaterials. Articles that synthesize information from multiple fields, and which place discoveries within a broader context, will be preferred. There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental or methodical details, or both, must be provided for research articles. Computed data or files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material. Nanomaterials is dedicated to a high scientific standard. All manuscripts undergo a rigorous reviewing process and decisions are based on the recommendations of independent reviewers.