Tsrp1 Is a Novel Cyclic Diguanosine Monophosphate Effector that Plays a Role in the Organic-Solvent Tolerance of Rhodococcus ruber.

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Fan Zhang, Han Yu, Lei Ai, Yixin Hao, Ren Peng
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引用次数: 0

Abstract

Cyclic diguanosine monophosphate is a ubiquitous second messenger that regulates diverse cellular processes. Rhodococcus ruber SD3 has potential for use in removing environmental pollutants such as phenol and toluene. In this study, Tsrp1 was found to be a novel cyclic diguanosine monophosphate effector in this strain. The interaction between Tsrp1 and c-di-GMP was verified by surface plasmon resonance, and the dissociation constant was 64 ± 6.84 μM. Moreover, in comparison with the wild-type strain, the recombinant R. ruber SD3 strain, which exhibited elevated levels tsrp1 gene expression, demonstrated enhanced growth in the presence of toluene and phenol. Both recombinant R. ruber SD3 and the wild-type strain completely degraded toluene (0.3 g/l, 0.6 g/l and 0.9 g/l) and phenol (0.6 g/l, 0.8 g/l and 1.0 g/l) in 72 h. Furthermore, differential expression of key genes encoding transcription factors was identified based on the transcriptomic comparison between the two strains. This study is the first to describe a novel cyclic diguanosine monophosphate effector and its role in the characteristics of R. ruber SD3, which will shed new light on the mechanisms underlying the organic solvent tolerance of R. ruber SD3.

Tsrp1是一种新型环二鸟苷单磷酸效应物,在橡胶红球菌的有机溶剂耐受性中起作用。
环二氨基二磷酸是一种普遍存在的第二信使,调节多种细胞过程。橡胶红球菌SD3在去除苯酚和甲苯等环境污染物方面具有潜在的应用前景。在本研究中,发现Tsrp1是该菌株中一种新的环二氨基二磷酸效应物。通过表面等离子体共振验证了Tsrp1与c-di-GMP的相互作用,解离常数为64±6.84 μM。此外,与野生型菌株相比,tsrp1基因表达水平升高的重组橡胶橡胶SD3菌株在甲苯和苯酚的存在下表现出更强的生长。重组橡胶红霉SD3和野生型菌株在72 h内均能完全降解甲苯(0.3 g/l、0.6 g/l和0.9 g/l)和苯酚(0.6 g/l、0.8 g/l和1.0 g/l)。通过转录组学比较,鉴定了两菌株编码转录因子的关键基因的差异表达。本研究首次描述了一种新型环二氨基膦效应物及其在橡胶胶SD3特性中的作用,这将为橡胶胶SD3耐有机溶剂的机制提供新的思路。
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来源期刊
Journal of microbiology and biotechnology
Journal of microbiology and biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
5.50
自引率
3.60%
发文量
151
审稿时长
2 months
期刊介绍: The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.
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