Effects of a Bacillus-based direct-fed microbial on digestibility, ruminal in situ nutrient disappearance, microbiome, and fermentation parameters in forage-fed beef cattle.

IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Arnaldo C Limede, Rodrigo S Marques, Fernando A A Cidrini, Vinicius A Cruz, Amanda C M Queiroz, Iorrano A Cidrini, Iasmin M S A Farias, Aline N Rodrigues, Eric J Scholljegerdes, Izadora S Souza, Reinaldo F Cooke, Raphaele Gresse, Gisle Vestergaard, Bruno I Cappellozza
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引用次数: 0

Abstract

This experiment evaluated dry matter intake, ruminal in situ nutrient disappearance, total-tract apparent nutrient digestibility, microbiome, and ruminal fermentation parameters of crossbred beef cows offered forage-based diets and a Bacillus-based direct-fed microbial (DFM). Fifteen rumen-cannulated Angus × Hereford cows were used in a crossover design. Treatments consisted of forage-based diets plus 500 g of protein-mineral supplement mixed with 1) 3 g of a Bacillus-based DFM containing Bacillus licheniformis and B. subtilis (BAC; n = 15; 2.2 × 109 CFU of the mixture/g; Bovacillus, Novonesis, Lyngby, Denmark), or 2) without BAC (CON; n = 15). Each experimental period lasted 33 days with 30 days of wash-out between periods. Cows were fed daily with chopped grass-mixed hay [Idaho fescue (Festuca idahoensis) and Bluebunch wheatgrass (Pseudoroegneria spicata)]. From day 17 to 26, 5 g of titanium dioxide (TiO2) was dosed twice daily, at 12-h intervals, as an external marker of fecal output. From day 22 to 26, fecal spot samples were collected at 12-h intervals. On day 27, ruminal fluid was collected prior to feeding (0 h) and at 4, 8, 12, 16, and 20 h post-feeding to evaluate ruminal pH, volatile fatty acids (VFA) and NH3-N. From day 28 to 32, 4 g of grass-mixed hay was placed in Dacron bags and introduced through the cannulas for 0, 12, 24, 48, 72, and 96 h. A treatment effect was observed (P = 0.01) for DMI, which was greater for cows supplemented with BAC vs. CON. Cows supplemented with BAC tended (P > 0.06) to have greater DM and NDF disappearance vs. CON. A treatment effect was observed (P ≤ 0.02) for total-tract apparent DM and CP digestibility, which were greater for BAC vs. CON cows. Also, cows supplemented with BAC tended (P ≥ 0.06) to have a greater total-tract NDF and ADF digestibility vs. CON cohorts. Therefore, cows supplemented with BAC had greater (P < 0.01) amounts of DM, CP, NDF, and ADF digested vs. CON cows. A treatment × time effect was observed (P = 0.02) for ruminal pH, which increased at 16 and 20 h of collection for BAC vs. CON cows. Treatment effects were not (P ≥ 0.18) observed for VFA, N-NH3, and plasma concentrations of glucose, whereas plasma concentration of urea tended (P = 0.06) to be reduced for cows supplemented with BAC vs. CON. Hence, supplementation with a Bacillus-based DFM increased forage intake, the digestibility of DM and CP, and the amount of nutrients digested by rumen-cannulated cows receiving a forage-based diet.

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来源期刊
Journal of animal science
Journal of animal science 农林科学-奶制品与动物科学
CiteScore
4.80
自引率
12.10%
发文量
1589
审稿时长
3 months
期刊介绍: The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year. Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.
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