A retrospective analysis of canine, feline, and equine respiratory polymerase chain reaction panels performed at the New York State Animal Health Diagnostic Center (January-December 2023).

IF 1.6 2区农林科学 Q2 VETERINARY SCIENCES

Javma-journal of The American Veterinary Medical Association Pub Date : 2025-03-25 DOI:10.2460/javma.24.11.0755

Kimberly Snedden, Elisha Frye, Rachel Conklin, Melissa Aprea, Mark Rishniw, Manigandan Lejeune, Erin Goodrich

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引用次数: 0

Abstract

Objective: To analyze the results and metadata of canine, feline, and equine respiratory PCR panel assays performed at the New York State Animal Health Diagnostic Center and inform veterinary diagnostic sample submission.

Methods: This retrospective study reviewed laboratory data from routine sample submissions to the Animal Health Diagnostic Center for canine, feline, and equine respiratory PCR panels from January 1 through December 31, 2023. Associations were compared between variables using χ2 tests of independence or Fisher exact tests.

Results: A total of 1,902 canine, feline, and equine respiratory panels were performed (705 canine, 189 feline, and 1,008 equine). The most common pathogens were Mycoplasma cynos (canine [66%]), Mycoplasma felis (feline [60%]), and Streptococcus equi subsp equi (equine [39%]). The most common coinfections were canine parainfluenza virus and M cynos (canine), M felis and feline calicivirus (feline), and S equi subsp equi and equine rhinitis virus B (equine). Acute canine cases were more likely to be positive than chronic cases (OR, 2.7; 95% CI, 1.7 to 4.5), with viruses more common than bacteria (OR, 4.7; 95% CI, 2.6 to 8.2). Canine and feline upper respiratory samples had a higher probability of detecting bacteria (χ2 < 0.0001; OR, 1.6; 95% CI, 1.3 to 2.1) than viruses (χ2 < 0.04; OR, 2.1; 95% CI, 1.1 to 3.9). There was a lower probability of detecting bacteria than viruses in equine upper respiratory samples (χ2 < 0.0001; OR, 0.4; 95% CI, 0.33 to 0.57).

Conclusions: This study highlights the probable pathogens detected in select respiratory panel PCR testing. Timing of sample collection during the respiratory illness may influence pathogen detection.

Clinical relevance: Acutely collected samples and clinically relevant information should be provided to laboratories to maximize diagnostic outcomes.

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来源期刊

Javma-journal of The American Veterinary Medical Association 农林科学-兽医学

CiteScore

1.60

自引率

15.80%

发文量

539

审稿时长

6-16 weeks

期刊介绍： Published twice monthly, this peer-reviewed, general scientific journal provides reports of clinical research, feature articles and regular columns of interest to veterinarians in private and public practice. The News and Classified Ad sections are posted online 10 days to two weeks before they are delivered in print.