Genetic association of long non-coding RNA ANRIL polymorphism with the risk of type 2 diabetes mellitus in the Chinese Han population.

IF 3.4 3区医学 Q2 ENDOCRINOLOGY & METABOLISM

Diabetology & Metabolic Syndrome Pub Date : 2025-03-28 DOI:10.1186/s13098-025-01670-3

Xinyi Li, Aige Yang, Xiao Liu, Rui Zhang, Huimin Zhou, Shunjiang Xu

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引用次数: 0

Abstract

Background: Type 2 diabetes mellitus (T2DM) is closely associated with both environmental and genetic factors, involving multi-gene inheritance. This study examined the association between the polymorphic locus rs10757278 in long non-coding RNA ANRIL and T2DM.

Methods: Polymerase chain reaction (PCR) was used to detect the rs10757278 polymorphism in the ANRIL gene. RT-qPCR measured ANRIL expression levels, and logistic regression identified independent risk factors for T2DM. Furthermore, the receiver operating characteristic (ROC) curve was constructed to evaluate the clinical diagnostic value of serum ANRIL levels in diagnosing T2DM.

Results: The rs10757278 polymorphism of the ANRIL was associated with the development of T2DM. Specifically, the G allele increases the risk of T2DM, and individuals carrying the GG genotype have a higher risk of developing the disease. Significant differences were found in low-density lipoprotein cholesterol (LDL-C), fasting plasma glucose (FPG), and glycated hemoglobin (HbA1c) among T2DM patients with different genotypes of ANRIL rs10757278. The relative FPG and HbA1c levels were relatively lower in individuals with the AA genotype and higher in those with the GG genotype. Moreover, serum ANRIL levels in the T2DM group were lower than in the control group. Body mass index (BMI), the rs10757278 locus, and serum ANRIL levels were independent risk factors for the development of T2DM. The ROC curve showed that serum ANRIL levels have significant clinical diagnostic value for the diagnosis of T2DM.

Conclusion: The rs10757278 polymorphism in ANRIL was strongly associated with the genetic predisposition to T2DM.

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背景：2型糖尿病（T2DM）与环境和遗传因素密切相关，涉及多基因遗传。本研究探讨了长非编码 RNA ANRIL 的多态性位点 rs10757278 与 T2DM 的关系：方法：采用聚合酶链反应（PCR）检测 ANRIL 基因中的 rs10757278 多态性。RT-qPCR测定了ANRIL的表达水平，逻辑回归确定了T2DM的独立风险因素。此外，还构建了接收者操作特征曲线（ROC），以评估血清 ANRIL 水平在诊断 T2DM 中的临床诊断价值：结果：ANRIL的rs10757278多态性与T2DM的发生有关。具体而言，G 等位基因会增加 T2DM 的发病风险，而 GG 基因型携带者的发病风险更高。在 ANRIL rs10757278 不同基因型的 T2DM 患者中，低密度脂蛋白胆固醇（LDL-C）、空腹血浆葡萄糖（FPG）和糖化血红蛋白（HbA1c）存在显著差异。AA 基因型患者的 FPG 和 HbA1c 水平相对较低，而 GG 基因型患者的 FPG 和 HbA1c 水平相对较高。此外，T2DM 组的血清 ANRIL 水平低于对照组。体重指数（BMI）、rs10757278位点和血清ANRIL水平是T2DM发病的独立风险因素。ROC曲线显示，血清ANRIL水平对T2DM的诊断具有显著的临床诊断价值：结论：ANRIL的rs10757278多态性与T2DM的遗传易感性密切相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diabetology & Metabolic Syndrome ENDOCRINOLOGY & METABOLISM-

CiteScore

6.20

自引率

0.00%

发文量

170

审稿时长

7.5 months

期刊介绍： Diabetology & Metabolic Syndrome publishes articles on all aspects of the pathophysiology of diabetes and metabolic syndrome. By publishing original material exploring any area of laboratory, animal or clinical research into diabetes and metabolic syndrome, the journal offers a high-visibility forum for new insights and discussions into the issues of importance to the relevant community.