Effects of EDTA, fumaric acid, and the chitosan solutions prepared with distilled water and acetic acid on dentin microhardness and tubular penetration: An in vitro study.

IF 2.7 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Esma Sariçam, Hanife Altınışık, Neslihan Bulak, Berna Güven, Sabiha Zeynep Yurdagül, Gülin Amasya
{"title":"Effects of EDTA, fumaric acid, and the chitosan solutions prepared with distilled water and acetic acid on dentin microhardness and tubular penetration: An in vitro study.","authors":"Esma Sariçam, Hanife Altınışık, Neslihan Bulak, Berna Güven, Sabiha Zeynep Yurdagül, Gülin Amasya","doi":"10.17219/dmp/167467","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Chitosan prepared with acetic acid is commonly used as an endodontic irrigant. However, the chitosan solution prepared with distilled water has not been evaluated for endodontic usage.</p><p><strong>Objectives: </strong>The present study aimed to compare the effects of ethylenediaminetetraacetic acid (EDTA), fumaric acid, and the chitosan solutions prepared with distilled water (C-DW) and acetic acid (C-AA) on dentin microhardness and dentinal tubule penetration.</p><p><strong>Material and methods: </strong>Eighty maxillary central incisors were endodontically instrumented and randomly divided into 2 main groups (n = 40) for the evaluation of dentin microhardness and tubular penetration, with 4 subgroups in each main group (n = 10) according to the final irrigation solutions used (EDTA, fumaric acid, C-DW, and C-AA). The C-AA solution was prepared by diluting medium-molecularweight chitosan in acetic acid. The C-DW solution was prepared with distilled water and chitosan ammonium salts, which were synthesized using trichloroacetic acid and low-molecular-weight chitosan. After irrigation, the roots were sectioned horizontally 2 mm (the apical third) and 5 mm (the middle third) from the apex. The microhardness measurements were taken at depths of 500 μm and 1,000 μm from the canal lumen. The sections were examined for tubular penetration using confocal laser scanning microscopy. The data was analyzed using the analysis of variance (ANOVA), with a significance level set at p < 0.05.</p><p><strong>Results: </strong>The microhardness values were statistically similar at either depth for each third (p > 0.05), except for the 1,000-μm depth in the apical third, where the use of the C-AA solution resulted in lower microhardness as compared to fumaric acid (p < 0.05). No significant differences were observed in tubular penetration with regard to each third (p > 0.05).</p><p><strong>Conclusions: </strong>All solutions showed a similar penetration ability in each third. At the 1,000-μm depth in the apical third, the fumaric acid solution provided a higher microhardness value than the C-AA solution.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Dental and Medical Problems","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17219/dmp/167467","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Chitosan prepared with acetic acid is commonly used as an endodontic irrigant. However, the chitosan solution prepared with distilled water has not been evaluated for endodontic usage.

Objectives: The present study aimed to compare the effects of ethylenediaminetetraacetic acid (EDTA), fumaric acid, and the chitosan solutions prepared with distilled water (C-DW) and acetic acid (C-AA) on dentin microhardness and dentinal tubule penetration.

Material and methods: Eighty maxillary central incisors were endodontically instrumented and randomly divided into 2 main groups (n = 40) for the evaluation of dentin microhardness and tubular penetration, with 4 subgroups in each main group (n = 10) according to the final irrigation solutions used (EDTA, fumaric acid, C-DW, and C-AA). The C-AA solution was prepared by diluting medium-molecularweight chitosan in acetic acid. The C-DW solution was prepared with distilled water and chitosan ammonium salts, which were synthesized using trichloroacetic acid and low-molecular-weight chitosan. After irrigation, the roots were sectioned horizontally 2 mm (the apical third) and 5 mm (the middle third) from the apex. The microhardness measurements were taken at depths of 500 μm and 1,000 μm from the canal lumen. The sections were examined for tubular penetration using confocal laser scanning microscopy. The data was analyzed using the analysis of variance (ANOVA), with a significance level set at p < 0.05.

Results: The microhardness values were statistically similar at either depth for each third (p > 0.05), except for the 1,000-μm depth in the apical third, where the use of the C-AA solution resulted in lower microhardness as compared to fumaric acid (p < 0.05). No significant differences were observed in tubular penetration with regard to each third (p > 0.05).

Conclusions: All solutions showed a similar penetration ability in each third. At the 1,000-μm depth in the apical third, the fumaric acid solution provided a higher microhardness value than the C-AA solution.

EDTA、富马酸和壳聚糖(蒸馏水和乙酸)对牙本质显微硬度和管状渗透的影响。
背景:乙酸制备壳聚糖是常用的牙髓冲洗剂。然而,蒸馏水制备的壳聚糖溶液在牙髓治疗中的应用尚未得到评价。目的:比较乙二胺四乙酸(EDTA)、富马酸以及用蒸馏水(C-DW)和乙酸(C-AA)配制的壳聚糖溶液对牙本质显微硬度和牙本质小管渗透性的影响。材料与方法:对80个上颌中切牙进行根管预备,随机分为2个主组(n = 40),评估牙本质显微硬度和管状穿透性,根据最终使用的冲洗液(EDTA、富马酸、C-DW、C-AA)分为4个亚组(n = 10)。用醋酸稀释中等分子量的壳聚糖制备C-AA溶液。以三氯乙酸和低分子量壳聚糖为原料,用蒸馏水和壳聚糖铵盐制备C-DW溶液。灌水后,根距根尖2 mm(顶端1 / 3)和5 mm(中间1 / 3)水平切片。分别在距管腔500 μm和1000 μm处测量显微硬度。切片用共聚焦激光扫描显微镜检查管状穿透。数据分析采用方差分析(ANOVA),显著性水平设置为p < 0.05。结果:每三分之一深度的显微硬度值在统计学上相似(p > 0.05),除了顶端1000 μm深度的三分之一,使用C-AA溶液导致显微硬度低于富马酸(p < 0.05)。每三分之一的管状穿透性无显著差异(p < 0.05)。结论:所有溶液在每三分之一的渗透能力相似。在顶端三分之一处1000 μm深度,富马酸溶液的显微硬度值高于C-AA溶液。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
4.00
自引率
3.80%
发文量
58
审稿时长
53 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信