Serum-Based Proteomic Approach to Identify Clinical Biomarkers of Radiation Exposure.

IF 4.5 2区医学 Q1 ONCOLOGY

Cancers Pub Date : 2025-03-17 DOI:10.3390/cancers17061010

Emeshaw Damtew Zebene, Biagio Pucci, Rita Lombardi, Hagos Tesfay Medhin, Edom Seife, Elena Di Gennaro, Alfredo Budillon, Gurja Belay Woldemichael

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引用次数: 0

Abstract

Background: Ionizing radiation (IR) exposure poses a significant health risk due to its widespread use in medical diagnostics and therapeutic applications, necessitating rapid and effective biomarkers for assessment.

Objective: The aim of this study is to identify the serum proteomic signature of IR exposure in patients undergoing radiotherapy (RT).

Methods: Blood samples were obtained from eighteen patients with head and neck cancer (HNC) and five patients with rectal cancer before and immediately after they underwent curative intensity-modulated radiotherapy (IMRT). The comprehensive serum proteome was analyzed in individual samples using nanoHPLC-MS/MS.

Results: Forty radiation-modulated proteins (RMPs), 24 upregulated and 16 downregulated, with a fold change ≥1.5 and p-value < 0.05 were identified. About 40% of the RMPs are involved in acute phase response, DNA repair, and inflammation; the key RMPs were ADCY1, HGF, MCEMP1, CHD4, RECQL5, MSH6, and ZNF224. Conclusions: This study identifies a panel of serum proteins that may reflect the radiation response, providing a valuable molecular fingerprint of IR exposure and paving the way for the development of sensitive and specific biomarkers for early detection and clinical management of IR-related injuries.

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基于血清的蛋白质组学方法识别辐射暴露的临床生物标记物

背景：电离辐射（IR）暴露由于其在医学诊断和治疗应用中的广泛应用而造成重大的健康风险，需要快速有效的生物标志物进行评估。目的：本研究的目的是确定放射治疗（RT）患者IR暴露的血清蛋白质组学特征。方法：对18例头颈癌（HNC）患者和5例直肠癌患者进行调强放疗（IMRT）治疗前后的血液标本进行分析。采用纳米高效液相色谱-质谱联用技术对单个样品进行综合血清蛋白质组分析。结果：共鉴定出40个辐射调节蛋白（RMPs），其中上调24个，下调16个，倍数变化≥1.5，p值< 0.05。约40%的RMPs参与急性期反应、DNA修复和炎症；关键RMPs为ADCY1、HGF、MCEMP1、CHD4、RECQL5、MSH6、ZNF224。结论：本研究确定了一组可能反映辐射反应的血清蛋白，为红外暴露提供了有价值的分子指纹，并为开发敏感和特异性的生物标志物铺平了道路，用于红外相关损伤的早期检测和临床管理。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancers Medicine-Oncology

CiteScore

8.00

自引率

9.60%

发文量

5371

审稿时长

18.07 days

期刊介绍： Cancers (ISSN 2072-6694) is an international, peer-reviewed open access journal on oncology. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.