Integration of transcriptomic and metabolomic analysis reveals light-regulated anthocyanin accumulation in the peel of 'Yinhongli' plum.

IF 4.3 2区 生物学 Q1 PLANT SCIENCES
Bo Xiong, Yisong Li, Junfei Yao, Jialu Wang, Linlyu Han, Qingqing Ma, Taimei Deng, Ling Liao, Lijun Deng, Guochao Sun, Mingfei Zhang, Xun Wan, Siya He, Jiaxian He, Zhihui Wang
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引用次数: 0

Abstract

Background: The 'Yinhongli' cultivar of Chinese plum (Prunus salicina Lindl.) is characterized by a distinctive bicolored peel phenotype, in which anthocyanins serve as crucial determinants of both its visual characteristics and nutritional quality. However, the molecular mechanism of underlying light-dependent anthocyanin biosynthesis of plum, especially its regulatory network and pathway, need to be further studied and explored.

Results: Comprehensive physiological analyses demonstrated distinct pigmentation patterns, revealing that dark-treated (YD) plum peels retained green coloration, whereas light-exposed (YL) and bag-removed samples (YDL) exhibited red pigmentation. Utilizing an integrated approach combining metabolomic and transcriptomic analyses, we identified 266 differentially accumulated flavonoids (DAFs), among which seven anthocyanin metabolites were established as principal determinants of peel coloration. Transcriptomic profiling revealed 6,900 differentially expressed genes (DEGs) between YD and YL, demonstrating significant correlations between the phenylpropanoid and flavonoid biosynthetic pathways. Through Weighted Gene Co-expression Network Analysis (WGCNA) and correlation heatmap analysis, we identified crucial regulatory networks encompassing five structural genes (PAL, 4CL, F3'H, CHI, and UFGT) and 15 candidate regulatory genes, including six light signal transduction factor genes (UVR8, COP1, PHYBs, PIF3, and HY5) and nine transcription factor genes (MYB1, MYB20, MYB73, MYB111, LHY, DRE2B, ERF5, bHLH35, and NAC87). Subsequent RT-qPCR validation demonstrated significant light-mediated up-regulation of key structural genes (PAL, F3H, CHI, 4CL, and UFGT) involved in anthocyanin biosynthesis along with positive regulatory factors (DRE2B and NAC87). Conversely, a cohort of negative regulators, including HY5, MYB1, MYB20, MYB73, MYB111, LHY, ERF5, and bHLH35, showed marked down-regulation in response to light exposure, suggesting their potential repressive roles in the light-dependent anthocyanin biosynthesis pathway.

Conclusions: This investigation provides comprehensive insights into the molecular mechanisms of anthocyanin biosynthesis in light-dependent anthocyanin biosynthesis in 'Yinhongli' plum, identifying critical structural genes and potential regulatory TFs. The findings offer substantial contributions to the understanding of anthocyanin regulation in fruit crops and provide a valuable foundation for molecular breeding initiatives aimed at enhancing quality traits in plum cultivars.

转录组和代谢组分析的整合揭示了'殷红李'果皮中受光调节的花青素积累。
背景:中国李(Prunus salicina Lindl.)的“Yinhongli”品种具有独特的双色果皮表型,其中花青素是其视觉特征和营养品质的关键决定因素。然而,李树光依赖性花青素生物合成的分子机制,特别是其调控网络和途径,还需要进一步研究和探索。结果:综合生理分析显示了不同的色素沉着模式,揭示了暗处理(YD)李子果皮保留绿色,而光暴露(YL)和去袋样品(YDL)呈现红色色素沉着。利用代谢组学和转录组学分析相结合的综合方法,我们鉴定了266种差异积累的黄酮类化合物(daf),其中7种花青素代谢物被确定为果皮颜色的主要决定因素。转录组学分析显示,YD和YL之间存在6900个差异表达基因(DEGs),表明苯丙素和类黄酮生物合成途径之间存在显著相关性。通过加权基因共表达网络分析(WGCNA)和相关热图分析,我们确定了关键的调控网络,包括5个结构基因(PAL、4CL、F3'H、CHI和UFGT)和15个候选调控基因,包括6个光信号转导因子基因(UVR8、COP1、PHYBs、PIF3和HY5)和9个转录因子基因(MYB1、MYB20、MYB73、MYB111、LHY、DRE2B、ERF5、bHLH35和NAC87)。随后的RT-qPCR验证表明,参与花青素生物合成的关键结构基因(PAL、F3H、CHI、4CL和UFGT)和光介导的显著上调以及正调节因子(DRE2B和NAC87)。相反,一系列负调节因子,包括HY5、MYB1、MYB20、MYB73、MYB111、LHY、ERF5和bHLH35,在光照下表现出明显的下调,表明它们在光依赖性花青素生物合成途径中具有潜在的抑制作用。结论:本研究全面揭示了银红里梅光依赖性花青素生物合成过程中花青素生物合成的分子机制,确定了关键结构基因和潜在的调控因子。研究结果为进一步了解果实作物花青素的调控机制提供了重要依据,并为提高李子品种品质性状的分子育种工作提供了有价值的基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Plant Biology
BMC Plant Biology 生物-植物科学
CiteScore
8.40
自引率
3.80%
发文量
539
审稿时长
3.8 months
期刊介绍: BMC Plant Biology is an open access, peer-reviewed journal that considers articles on all aspects of plant biology, including molecular, cellular, tissue, organ and whole organism research.
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