The Influence of Aspiration Pressure, Follicle Flushing Method and Needle Rotation During Single-Operator OPU Technique on Oocyte Recovery and Embryo Production in the Mare.

Abstract

The objective of this study was to determine the effect of two aspiration pressures (75 vs. 150 mmHg), the follicle flushing method (injection pump controlled by a foot pedal vs. a plastic syringe) and the twisting of the OPU needle on oocyte recovery and in vitro embryo production. OPU data from a total of 104 warmblood sport mares belonging to a commercial OPU-ICSI program were collected as part of a prospective study split into three experiments. Each mare was used only once for OPU. In Experiment 1, the mares' follicles were aspirated using either a high aspiration pressure (flow rate of 1.33 mL/s; n = 18) or low aspiration pressure (0.75 mL/s; n = 18); in Experiment 2, follicles were flushed using either a manual method (plastic syringe, n = 18) or an automatic method (injection pump controlled by a foot pedal, n = 18); and in Experiment 3, the follicles were aspirated by scraping the follicle wall with needle rotation (needle twisting, n = 16) or without needle rotation (control, n = 16). In all the experiments, the same OPU operator and technician searching oocytes were used, and the allocation of each mare to the different treatment groups was randomized. The overall mean oocyte recovery rate of the study was 54.2 ± 17.1%, and the mean number of embryos per OPU-ICSI session was 1.9 ± 1.6. The oocyte recovery rate was not influenced by any of the parameters investigated (p > 0.05). However, high aspiration pressure (150 mmHg) tended to yield oocytes with lower maturation (51.6%; p = 0.09) and blastocyst rates (20.6%; p = 0.08) following IVM and ICSI, respectively, compared with the low aspiration group (64.4% MII rate and 31.4% blastocyst rate). In conclusion, increasing aspiration pressure does not increase oocyte recovery. Furthermore, when a single operator performs the OPU (holding the ovary and handling the needle simultaneously), needle rotation to scrape the follicle wall does not improve oocyte recovery.