Characterization of Canine Otitis Externa Pseudomonas aeruginosa Isolates and Their Sensitivities to Different Essential Oils.

IF 2.7 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animals Pub Date : 2025-03-13 DOI:10.3390/ani15060826

Anita Seres-Steinbach, Brigitta Schneider-Patkó, Ákos Jerzsele, Adrienn Mercedesz Veres, Ágnes Sonnevend, Krisztián Bányai, György Schneider

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Abstract

Otitis externa is common infection in dogs. Its most important aetiologic agent is Pseudomonas aeruginosa, which, besides its ability to form biofilm, can also be resistant to several antibiotics, leading to therapeutic failures in several cases. Promising therapeutic alternatives are constantly being sought to treat chronic, recurrent infections. Within the framework of this study, we investigated the antibacterial potential of essential oils against canine isolates of P. aeruginosa. Forty P. aeruginosa were isolated from individual dogs' otitis externa. They were identified by MALDI-TOF MS, their antibiotic susceptibility was determined with disk diffusion, and their genetic relatedness was established using pulsed-field gel electrophoresis. Fifty-seven essential oils were screened for their antipseudomonas effects using the drop plate method, and six essential oils-cinnamon (bark and leaf), bay, rosemary, thyme, and bitter orange-were further tested for their mature-biofilm-degrading capacities at 30 °C and 37 °C. Even though the molecular relatedness of the forty P. aeruginosa isolates could be excluded, all isolates' growth was inhibited and their biofilms degraded by a 2.5% concentration of cinnamon (bark and leaf), bay, rosemary, thyme, or bitter orange essential oil. Our results show that essential oils are potent alternatives in the treatment of otitis externa.

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来源期刊

Animals Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

4.90

自引率

16.70%

发文量

3015

审稿时长

20.52 days

期刊介绍： Animals (ISSN 2076-2615) is an international and interdisciplinary scholarly open access journal. It publishes original research articles, reviews, communications, and short notes that are relevant to any field of study that involves animals, including zoology, ethnozoology, animal science, animal ethics and animal welfare. However, preference will be given to those articles that provide an understanding of animals within a larger context (i.e., the animals'' interactions with the outside world, including humans). There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental details and/or method of study, must be provided for research articles. Articles submitted that involve subjecting animals to unnecessary pain or suffering will not be accepted, and all articles must be submitted with the necessary ethical approval (please refer to the Ethical Guidelines for more information).