Enhanced Thermal Stability/Activity of Geobacillus jurassicus Esterase by Rational Design and Application in the Synthesis of Cinnamyl Acetate.

IF 3.1 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Junze Li, Runfei Song, Lin Lin, Tao Li, Yan Yan, Wei Wei, Dongzhi Wei
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引用次数: 0

Abstract

Geobacillus sp. represents an important source of thermophilic esterases, yet studies on the rational design and industrial application of these enzymes remain limited. In our previous research, we identified the esterase Gju768 from Geobacillus jurassicus DSMZ 15726. In the present study, we employed a novel computer-aided rational design approach, ACDP (AutoDock, Consurf, Discovery Studio, PoPMuSiC), to enhance the enzyme's thermal stability. Through molecular docking and conservation analysis, three hotspots were identified. Virtual saturation mutagenesis was subsequently performed, yielding two selected mutations, Q78I and Q78L, from the resulting library. Notably, mutants Q78I and Q78L exhibited significant improvements in thermal stability and enzyme activity compared to the wild type (WT). Compared to WT, mutants Q78I and Q78L exhibited a 65.27% and 38.38% increase in half-life at 65 °C, along with a 14.48% and 1.60% improvement in specific activity at their respective optimal temperatures. Furthermore, under optimized conditions for cinnamyl acetate production, mutant Q78I demonstrated a yield of 68%, compared to only 31% for WT. This study underscored the potential of protein engineering strategies to enhance enzyme performance in industrial applications, particularly for the synthesis of value-added compounds such as cinnamyl acetate.

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来源期刊
Applied Biochemistry and Biotechnology
Applied Biochemistry and Biotechnology 工程技术-生化与分子生物学
CiteScore
5.70
自引率
6.70%
发文量
460
审稿时长
5.3 months
期刊介绍: This journal is devoted to publishing the highest quality innovative papers in the fields of biochemistry and biotechnology. The typical focus of the journal is to report applications of novel scientific and technological breakthroughs, as well as technological subjects that are still in the proof-of-concept stage. Applied Biochemistry and Biotechnology provides a forum for case studies and practical concepts of biotechnology, utilization, including controls, statistical data analysis, problem descriptions unique to a particular application, and bioprocess economic analyses. The journal publishes reviews deemed of interest to readers, as well as book reviews, meeting and symposia notices, and news items relating to biotechnology in both the industrial and academic communities. In addition, Applied Biochemistry and Biotechnology often publishes lists of patents and publications of special interest to readers.
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