Song Yang, Shengyu Zhou, Quanfeng Liang, Yi Wang, Wei Luo
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引用次数: 0
Abstract
L-tryptophan is an essential amino acid widely used in animal feed, food additives, and pharmaceuticals. Although the biosynthesis of L-tryptophan from renewable raw materials is sustainable and environmentally friendly, its long synthetic pathway and complex regulatory mechanisms often result in low production efficiency. In this study, multiple strategies were employed to improve L-tryptophan production in Escherichia coli. These strategies included three main ones: the introduction of anti-feedback inhibition mutations, the overexpression of key rate-limiting enzymes, and the blockage of competitive and catabolic pathways. Additionally, the glucose transport system was modified by replacing it with a non-phosphoenolpyruvate (PEP)-dependent system, thereby reducing PEP consumption. In addition, overexpression of an L-tryptophan transporter protein enhanced L-tryptophan efflux and alleviated product inhibition. Consequently, an efficient L-tryptophan-producing E. coli strain was constructed. Furthermore, dynamic regulation of L-tryptophan synthesis was initially explored by employing a temperature-sensitive promoter to modulate the PEP metabolic pathway. Scale-up fermentation in a 5-L bioreactor demonstrated that the final strain (TY9) produced 34.1 g·L-1 L-tryptophan under fed-batch conditions.
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This journal is devoted to publishing the highest quality innovative papers in the fields of biochemistry and biotechnology. The typical focus of the journal is to report applications of novel scientific and technological breakthroughs, as well as technological subjects that are still in the proof-of-concept stage. Applied Biochemistry and Biotechnology provides a forum for case studies and practical concepts of biotechnology, utilization, including controls, statistical data analysis, problem descriptions unique to a particular application, and bioprocess economic analyses. The journal publishes reviews deemed of interest to readers, as well as book reviews, meeting and symposia notices, and news items relating to biotechnology in both the industrial and academic communities.
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