A SlMYB78-regulated bifunctional gene cluster for phenolamide and salicylic acid biosynthesis during tomato domestication, reducing disease resistance.

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Peng Cao, Linghao Xia, Xianggui Li, Meng Deng, Zhonghui Zhang, Xiangyu Lin, Zeyong Wu, Yingchen Hao, Penghui Liu, Chao Wang, Chun Li, Jie Yang, Jun Lai, Jun Yang, Shouchuang Wang
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引用次数: 0

Abstract

Plants have evolved a sophisticated chemical defense network to counteract pathogens, with phenolamides and salicylic acid (SA) playing pivotal roles in the immune response. However, the synergistic regulatory mechanisms of their biosynthesis remain to be explored. Here, we identified a biosynthetic gene cluster on chromosome 2 (BGC2) associated with the biosynthesis of phenolamide and SA, wherein the key component SlEPS1 exhibits dual catalytic functions for the synthesis of phenolamides and SA. Overexpression of the key component SlEPS1 of BGC2 in tomato enhanced resistance to the bacterial pathogen Pst DC3000, whereas knockout plants were more susceptible. Exogenous applications of SA and phenolamides revealed that these two compounds act synergistically to enhance plant resistance. Notably, during tomato domestication, a disease-resistant allele of SlEPS1, SlEPS1HapB, was subject to negative selection, leading to a reduction in phenolamide and SA levels and compromised disease resistance in modern varieties. Moreover, the SlMYB78 directly regulates the BGC2 gene cluster to enhance phenolamide and SA biosynthesis, modulating resistance to Pst DC3000. Our study employed multi-omics approaches to describe the synergistic regulation of phenolamide and SA biosynthesis, offering new insights into the complexity of plant immune-related metabolism.

slmyb78调控的番茄驯化过程中酚酰胺和水杨酸生物合成双功能基因簇,降低抗病能力。
植物已经进化出一个复杂的化学防御网络来抵抗病原体,其中酚酰胺和水杨酸(SA)在免疫反应中起着关键作用。然而,其生物合成的协同调控机制仍有待探索。在这里,我们在2号染色体(BGC2)上发现了一个与酚酰胺和SA的生物合成相关的生物合成基因簇,其中关键组分SlEPS1对酚酰胺和SA的合成具有双重催化功能。BGC2关键成分SlEPS1的过表达增强了番茄对Pst DC3000的抗性,而敲除植株对Pst DC3000的抗性更强。外源应用SA和酚酰胺表明,这两种化合物协同作用,增强植物的抗性。值得注意的是,在番茄驯化过程中,SlEPS1的抗病等位基因SlEPS1HapB受到负选择的影响,导致现代品种的酚胺和SA水平降低,抗病性降低。此外,SlMYB78直接调控BGC2基因簇,增强酚胺和SA的生物合成,调节对Pst DC3000的抗性。我们的研究采用多组学方法描述了酚胺和SA生物合成的协同调节,为了解植物免疫相关代谢的复杂性提供了新的见解。
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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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