Analysis of Testosterone Esters in Serum and DBS Samples-Results From an Interlaboratory Study.

IF 2.6 3区 医学 Q2 BIOCHEMICAL RESEARCH METHODS
Tobias Langer, Alessandro Musenga, Biljana Stojanovic, Daniel Pecher, Günter Gmeiner, Laura Harju, Tina Suominen, Cynthia Mongongu, Magnus Ericsson, Silke Grabherr, Tiia Kuuranne, Raul Nicoli
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引用次数: 0

Abstract

Testosterone (T) formulations that are used for doping purposes often contain the steroid in esterified forms. As these esters are hydrolysed in the bloodstream before renal excretion, they can be detected in blood matrices and have not been detected in urine so far. Serum samples can additionally be used for longitudinal blood steroid profiling, but their collection, shipping and storage have some disadvantages. The use of dried blood spots (DBS), an alternative blood matrix, is more convenient for pre-analytical and post-analytical aspects but is not fully established in antidoping laboratories yet. To evaluate the ability of multiple antidoping laboratories to detect T-esters in serum and DBS samples, an interlaboratory study was organised. Common T-esters were spiked in five samples of each matrix (serum, cellulose card DBS, polymeric DBS) at concentrations that correspond to an administration scenario and sent as blinded specimens to each laboratory. The laboratories were requested to apply their own analytical method to detect the T-esters and to provide a rough estimate of their concentrations. All laboratories identified the spiked testosterone esters correctly in all samples and the estimated concentrations were deemed comparable (average relative standard deviation < 30%), considering that only qualitative initial testing procedures (ITPs) were used. This study could firstly demonstrate the capability of different analytical approaches to analyse T-esters in serum and DBS samples and, secondly, show that the methods employed by the participating laboratories are all fit for purpose.

血清和DBS样本中睾酮酯的分析——来自实验室间研究的结果。
用于兴奋剂目的的睾酮制剂通常含有酯化形式的类固醇。由于这些酯类物质在肾脏排泄前已在血液中水解,因此可在血液基质中检测到,而在尿液中尚未检测到。血清样本也可用于纵向血液类固醇分析,但其收集,运输和储存有一些缺点。使用干血点(DBS),一种替代的血液基质,在分析前和分析后更方便,但在反兴奋剂实验室尚未完全建立。为了评估多个反兴奋剂实验室检测血清和DBS样本中t -酯的能力,组织了一项实验室间研究。在每种基质(血清、纤维素卡DBS、聚合物DBS)的5个样品中按相应的浓度加标普通t -酯,并作为盲法标本送到每个实验室。要求实验室采用自己的分析方法来检测t -酯,并提供其浓度的粗略估计。考虑到只使用了定性初始测试程序(ITPs),所有实验室都正确地识别了所有样品中的添加睾酮酯,并且认为估计浓度具有可比性(平均相对标准偏差< 30%)。本研究首先证明了不同分析方法分析血清和DBS样品中t -酯的能力,其次表明参与实验室采用的方法都符合目的。
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来源期刊
Drug Testing and Analysis
Drug Testing and Analysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL
CiteScore
5.90
自引率
24.10%
发文量
191
审稿时长
2.3 months
期刊介绍: As the incidence of drugs escalates in 21st century living, their detection and analysis have become increasingly important. Sport, the workplace, crime investigation, homeland security, the pharmaceutical industry and the environment are just some of the high profile arenas in which analytical testing has provided an important investigative tool for uncovering the presence of extraneous substances. In addition to the usual publishing fare of primary research articles, case reports and letters, Drug Testing and Analysis offers a unique combination of; ‘How to’ material such as ‘Tutorials’ and ‘Reviews’, Speculative pieces (‘Commentaries’ and ‘Perspectives'', providing a broader scientific and social context to the aspects of analytical testing), ‘Annual banned substance reviews’ (delivering a critical evaluation of the methods used in the characterization of established and newly outlawed compounds). Rather than focus on the application of a single technique, Drug Testing and Analysis employs a unique multidisciplinary approach to the field of controversial compound determination. Papers discussing chromatography, mass spectrometry, immunological approaches, 1D/2D gel electrophoresis, to name just a few select methods, are welcomed where their application is related to any of the six key topics listed below.
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