Refinement of Synthetization Parameters for High Laccase-Like Activity of Imidazole-Copper (II) Nitrate Trihydrate Nanozyme Towards an Efficient Biomimetic Nanozyme.

IF 3.1 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Nur Aizura Mat Alewi, Roshanida A Rahman, Rosli Md Illias, Nardiah Rizwana Jaafar, Noor Hidayah Abd Rahman, Bee Jie Chia, Hui Lun Soo, Ariadne L Juwono, Munawar Khalil, Noverra M Nizardo
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Abstract

Laccase's industrial application is hindered by its sensitivity and low stability to extreme conditions. To overcome these limitations, the development of biomimetic nanozymes is gaining momentum. Nevertheless, developing multifunctional nanozymes with high laccase-like activity poses several challenges. This study focused on optimizing the synthesis of imidazole-copper (II) nitrate trihydrate (I-Cu) nanozymes and characterizing its physicochemical properties. Key synthesis parameters (precursor amount, incubation time, and oven temperature) were optimized. I-Cu nanozymes were synthesized in a Teflon-lined autoclave via water-induced precipitation of Cu2+ and imidazole, mimicking the N-Cu coordination found in laccase's active sites. Initial screenings revealed the superior catalytic activity of I-Cu nanozymes synthesized using methanol compared to ethanol, and a smaller nano-scale size than laccase. FTIR analysis confirmed the presence of similar chemical components as laccase (C44H69N11O20), verifying I-Cu nanozyme's capability to degrade phenolic compounds, and imidazole did not decompose throughout the synthesis process. The optimized I-Cu nanozyme demonstrated higher catalytic activity (6.569 UA), oxidation efficiency (Vmax of 0.00893 mM/min and Km of 2.4020 mM), and greater stability under varying pH, temperature, and storage conditions, compared to laccase. Conclusively, the optimized I-Cu nanozyme, with a 6.00-fold increase in catalytic activity compared to previous studies, as well as 1.69-fold higher Km, and 2.08-fold higher Vmax compared to laccase, shows promise as a wastewater treatment alternative. Its enhanced performance, achieved with fewer precursors through synthesis optimization, highlights the potential of lesser-known biomimetic nanozymes and underscores the importance of refining the synthesis parameters.

漆酶对极端条件的敏感性和低稳定性阻碍了它在工业上的应用。为了克服这些局限性,生物仿生纳米酶的发展势头日益强劲。然而,开发具有高漆酶活性的多功能纳米酶面临着一些挑战。本研究的重点是优化咪唑-三水硝酸铜(I-Cu)纳米酶的合成及其理化性质。研究优化了关键合成参数(前体量、孵育时间和烘箱温度)。I-Cu 纳米酶是在特氟隆内衬高压釜中通过水诱导 Cu2+ 和咪唑沉淀合成的,模仿了漆酶活性位点中的 N-Cu 配位。初步筛选显示,使用甲醇合成的 I-Cu 纳米酶的催化活性优于使用乙醇合成的 I-Cu 纳米酶,而且纳米尺寸小于漆酶。傅立叶变换红外分析证实,I-Cu 纳米酶中存在与漆酶类似的化学成分(C44H69N11O20),验证了 I-Cu 纳米酶降解酚类化合物的能力,而且咪唑在整个合成过程中都没有分解。与漆酶相比,优化后的 I-Cu 纳米酶具有更高的催化活性(6.569 UA)、氧化效率(Vmax 为 0.00893 mM/min,Km 为 2.4020 mM)以及在不同 pH 值、温度和储存条件下的稳定性。总之,优化后的 I-Cu 纳米酶的催化活性比以前的研究提高了 6.00 倍,Km 和 Vmax 分别比漆酶高出 1.69 倍和 2.08 倍,有望成为废水处理的替代品。这种催化剂通过合成优化,以更少的前体实现了更高的性能,突出了鲜为人知的仿生纳米酶的潜力,并强调了完善合成参数的重要性。
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来源期刊
Applied Biochemistry and Biotechnology
Applied Biochemistry and Biotechnology 工程技术-生化与分子生物学
CiteScore
5.70
自引率
6.70%
发文量
460
审稿时长
5.3 months
期刊介绍: This journal is devoted to publishing the highest quality innovative papers in the fields of biochemistry and biotechnology. The typical focus of the journal is to report applications of novel scientific and technological breakthroughs, as well as technological subjects that are still in the proof-of-concept stage. Applied Biochemistry and Biotechnology provides a forum for case studies and practical concepts of biotechnology, utilization, including controls, statistical data analysis, problem descriptions unique to a particular application, and bioprocess economic analyses. The journal publishes reviews deemed of interest to readers, as well as book reviews, meeting and symposia notices, and news items relating to biotechnology in both the industrial and academic communities. In addition, Applied Biochemistry and Biotechnology often publishes lists of patents and publications of special interest to readers.
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