Engineered Assemblies from Constitutionally Isomeric Peptides Modulate Antimicrobial Activity.

IF 5.5 2区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yujia Lu, Guanyi Li, Yanwen Zhang, Yuxuan Ge, Bin Hao, Yu Yin, Yaxue Zhao, Yin Wang
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引用次数: 0

Abstract

Antimicrobial peptides (AMPs) are a class of peptides consisting of cationic amino acid residues and a hydrophobic segment, which have been used as an alternative to antibiotics in treating multidrug-resistant bacteria. However, the relationship among the molecular design, assembled structures, and resultant efficacy remains elusive. Herein, we report a class of constitutionally isomeric AMPs assembled into filaments with similar dimensions. Spectroscopic characterizations demonstrated that subtle changes in the position of amino acids led to dramatic variations in molecular packing and surface charges, which were verified by molecular dynamics simulations. In vitro antibacterial assays showed that all AMPs exerted antibacterial activity against Gram-positive methicillin-resistant Staphylococcus aureus (MRSA), but the efficacy was dependent on the molecular design. Given the good biocompatibility to eukaryotic cells, these AMPs could be potentially used as antibacterial agents. We believe that this finding provides an avenue to tune the bioactivity of AMPs by rational molecular design.

结构异构肽的工程组装调节抗菌活性。
抗菌肽(Antimicrobial peptides, AMPs)是一类由阳离子氨基酸残基和疏水片段组成的肽,已被用作抗生素的替代品,用于治疗多重耐药细菌。然而,分子设计、组装结构和由此产生的功效之间的关系仍然是难以捉摸的。在此,我们报告了一类结构上异构的amp组装成具有相似尺寸的细丝。光谱表征表明,氨基酸位置的细微变化导致了分子堆积和表面电荷的剧烈变化,这一点得到了分子动力学模拟的证实。体外抗菌实验表明,所有抗菌肽均对革兰氏阳性耐甲氧西林金黄色葡萄球菌(MRSA)具有抗菌活性,但其抑菌效果与分子设计有关。由于对真核细胞具有良好的生物相容性,这些amp具有潜在的抗菌潜力。我们相信这一发现为通过合理的分子设计来调节amp的生物活性提供了一条途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biomacromolecules
Biomacromolecules 化学-高分子科学
CiteScore
10.60
自引率
4.80%
发文量
417
审稿时长
1.6 months
期刊介绍: Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine. Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.
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