Nuclear Staining and Fluorogenic Probe–Based Evaluation of ROS-Cytotoxic Potential Using AgNPs Synthesized From Cajanus trinervius Against A431 Cell Line

IF 3.2 4区 化学 Q2 CHEMISTRY, ANALYTICAL
Luminescence Pub Date : 2025-03-29 DOI:10.1002/bio.70146
Jayanthi Kamalanathan, M. S. Suresh, Venkatesh Rajendran, Mohamad S. AlSalhi, Sandhanasamy Devanesan, Arumugam Suresh, Jerrine Joseph, Shobana Sampath
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Abstract

The purpose of this study was to analyse the probable phytochemical compounds that were in the leaf extract of a shrub known as Cajanus trinervius (CT). We achieved this by using the gas chromatography–mass spectrometry (GC-MS) method in polar solvents like methanol, personal ether and ethanol. We evaluated the crude sample in a variety of solvents and found several distinct chemicals, including phenolics, terpenoids, flavonoids, carbohydrates, amino acids, glycosides, proteins, steroids, tannins and alkaloids. According to the outcomes of the GC-MS analysis, the mediums containing methanol, ethanol and pet ether each included 78, 82 and 38 chemical components, respectively. Sesquiterpenoids, phenolic compounds, amino substances and long-chain hydrocarbons were the most abundant chemicals. The outcomes of the experiment, along with the presence of a variety of phytochemicals in this plant, encouraged us to carry out the microwave synthesis of silver nanoparticles from this leaf extract and analyse them. We used the MTT test on the A431 cell line, to assess the damaging effects of aqueous CT extracts and CT-Ag NPs. Dual and DAPI staining confirmed the apoptotic activity of Cajanus trinervius leaf extract along with their ROS release assessment confirmation by DCFDA staining.

Abstract Image

本研究的目的是分析一种名为 Cajanus trinervius(CT)的灌木叶提取物中可能存在的植物化学化合物。为此,我们在甲醇、乙醚和乙醇等极性溶剂中使用了气相色谱-质谱法(GC-MS)。我们在各种溶剂中对粗样品进行了评估,并发现了几种不同的化学物质,包括酚类、萜类、黄酮类、碳水化合物、氨基酸、苷类、蛋白质、类固醇、单宁酸和生物碱。根据气相色谱-质谱分析结果,含有甲醇、乙醇和石油醚的培养基中分别含有 78、82 和 38 种化学成分。半萜、酚类化合物、氨基酸和长链烃类是含量最高的化学物质。实验结果以及该植物中多种植物化学物质的存在,促使我们用这种叶提取物进行微波合成银纳米粒子并对其进行分析。我们使用 MTT 测试 A431 细胞系,评估 CT 水提取物和 CT-Ag NPs 的破坏作用。双重染色和 DAPI 染色证实了 Cajanus trinervius 叶提取物的凋亡活性,DCFDA 染色也证实了它们的 ROS 释放评估。
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来源期刊
Luminescence
Luminescence 生物-生化与分子生物学
CiteScore
5.10
自引率
13.80%
发文量
248
审稿时长
3.5 months
期刊介绍: Luminescence provides a forum for the publication of original scientific papers, short communications, technical notes and reviews on fundamental and applied aspects of all forms of luminescence, including bioluminescence, chemiluminescence, electrochemiluminescence, sonoluminescence, triboluminescence, fluorescence, time-resolved fluorescence and phosphorescence. Luminescence publishes papers on assays and analytical methods, instrumentation, mechanistic and synthetic studies, basic biology and chemistry. Luminescence also publishes details of forthcoming meetings, information on new products, and book reviews. A special feature of the Journal is surveys of the recent literature on selected topics in luminescence.
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