Three-dimensional tracking of microswimmer suspensions

IF 2.3 3区 工程技术 Q2 ENGINEERING, MECHANICAL
Junaid Mehmood, Koen Muller, Sowmya Kumar, Abel-John Buchner, Daniel Tam
{"title":"Three-dimensional tracking of microswimmer suspensions","authors":"Junaid Mehmood,&nbsp;Koen Muller,&nbsp;Sowmya Kumar,&nbsp;Abel-John Buchner,&nbsp;Daniel Tam","doi":"10.1007/s00348-025-04002-3","DOIUrl":null,"url":null,"abstract":"<div><p>Understanding the locomotion of microorganisms is essential for insights into microbial ecology, infection, and colonization processes. Although two-dimensional microscopy has been widely used to study microswimmer motility, it does not capture the full extent of their three-dimensional (3D) movement. Recent advances in defocused particle tracking, holographic tracking velocimetry, and stereo-microscopy face challenges in achieving high resolution at larger particle densities and tracking multiple microswimmers in suspension. In this work, we introduce a novel multi-camera microscopy system that significantly improves the accuracy of 3D microswimmer tracking. Our system uses four sCMOS cameras to image microorganisms within a 2.5 <span>\\(\\times\\)</span> 2.5 <span>\\(\\times\\)</span> 2 mm<span>\\(^3\\)</span>. We assess the performance of our microscopy system by tracking a population of the unicellular motile algae <i>Chlamydomonas reinhardtii</i>. An in-house tracking algorithm based on the projective geometry framework enables tracking with reprojection errors below 0.3 body lengths. This system supports imaging and tracking particle source densities of 0.32, higher than other existing single camera 3D microscopy techniques. Analysis of <i>C. reinhardtii</i> trajectories in 3D reveals a predominance of left-handed chirality and helical swimming patterns. Moreover, our 3D tracking data provide translational and rotational diffusion coefficients that differ from those obtained using traditional two-dimensional methods.</p></div>","PeriodicalId":554,"journal":{"name":"Experiments in Fluids","volume":"66 4","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00348-025-04002-3.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experiments in Fluids","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s00348-025-04002-3","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, MECHANICAL","Score":null,"Total":0}
引用次数: 0

Abstract

Understanding the locomotion of microorganisms is essential for insights into microbial ecology, infection, and colonization processes. Although two-dimensional microscopy has been widely used to study microswimmer motility, it does not capture the full extent of their three-dimensional (3D) movement. Recent advances in defocused particle tracking, holographic tracking velocimetry, and stereo-microscopy face challenges in achieving high resolution at larger particle densities and tracking multiple microswimmers in suspension. In this work, we introduce a novel multi-camera microscopy system that significantly improves the accuracy of 3D microswimmer tracking. Our system uses four sCMOS cameras to image microorganisms within a 2.5 \(\times\) 2.5 \(\times\) 2 mm\(^3\). We assess the performance of our microscopy system by tracking a population of the unicellular motile algae Chlamydomonas reinhardtii. An in-house tracking algorithm based on the projective geometry framework enables tracking with reprojection errors below 0.3 body lengths. This system supports imaging and tracking particle source densities of 0.32, higher than other existing single camera 3D microscopy techniques. Analysis of C. reinhardtii trajectories in 3D reveals a predominance of left-handed chirality and helical swimming patterns. Moreover, our 3D tracking data provide translational and rotational diffusion coefficients that differ from those obtained using traditional two-dimensional methods.

微游泳者悬架的三维跟踪
了解微生物的运动对于了解微生物生态学、感染和定植过程至关重要。虽然二维显微镜已被广泛用于研究微游泳者的运动,但它并不能捕捉到他们的三维运动的全部范围。离焦粒子跟踪、全息跟踪测速和立体显微镜的最新进展面临着在更大粒子密度下实现高分辨率和在悬浮中跟踪多个微游泳者的挑战。在这项工作中,我们介绍了一种新型的多相机显微镜系统,该系统显著提高了三维微游泳者跟踪的精度。我们的系统使用四个sCMOS相机对2.5 \(\times\) 2.5 \(\times\) 2 mm \(^3\)内的微生物进行成像。我们通过跟踪单细胞运动藻类莱茵衣藻的种群来评估显微镜系统的性能。基于投影几何框架的内部跟踪算法使跟踪的重投影误差低于0.3体长。该系统支持成像和跟踪密度为0.32的粒子源,高于其他现有的单相机3D显微镜技术。对莱因哈蒂瓢虫三维轨迹的分析揭示了左手手性和螺旋游动模式的优势。此外,我们的3D跟踪数据提供了不同于传统二维方法获得的平移和旋转扩散系数。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Experiments in Fluids
Experiments in Fluids 工程技术-工程:机械
CiteScore
5.10
自引率
12.50%
发文量
157
审稿时长
3.8 months
期刊介绍: Experiments in Fluids examines the advancement, extension, and improvement of new techniques of flow measurement. The journal also publishes contributions that employ existing experimental techniques to gain an understanding of the underlying flow physics in the areas of turbulence, aerodynamics, hydrodynamics, convective heat transfer, combustion, turbomachinery, multi-phase flows, and chemical, biological and geological flows. In addition, readers will find papers that report on investigations combining experimental and analytical/numerical approaches.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信