Scleral ultrastructure in young rhesus monkeys: A stereological approach

IF 1.5 4区心理学 Q4 NEUROSCIENCES

Vision Research Pub Date : 2025-03-28 DOI:10.1016/j.visres.2025.108592

Suharsha Paidimarri, Baskar Arumugam, Li-Fang Hung, Earl L. Smith III, Alan R. Burns, Lisa A. Ostrin

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引用次数: 0

Abstract

The sclera provides mechanical support to the globe and plays a key role in maintenance of ocular structural integrity. Evidence shows that scleral remodeling contributes to biomechanical weakening, increasing the risk of pathologies, such as glaucoma and posterior staphyloma in high myopia. A precise characterization of normal regional ultrastructure is needed for a better understanding of scleral remodeling. The purpose of this study was to develop and implement a stereological analysis method to characterize scleral ultrastructure in healthy young rhesus monkey eyes. Monkeys (N = 4 eyes) first underwent normal emmetropization in a primate nursery. At 150 days of age, scleral tissue was collected in 1 mm² sections from three regions, equatorial nasal, posterior, and equatorial temporal, and processed for transmission electron microscopy (TEM). Images were captured at three depths: outer, middle, and inner, for all regions. Scleral thickness, fibroblast volume fraction, volume fraction of fibrillin microfibrils, and collagen fibril diameters were quantified using stereology and manual segmentation. The sclera was thickest in the posterior region with a mean thickness of 217.1 ± 10.1 μm. A total of 103 micrographs were analyzed to compute the volume fraction of fibroblasts and fibrillin microfibrils. Median fibroblast volume fraction was 7.4 %, and fibrillin microfibril volume fraction was 1.8 %. A total of 3564 collagen fibrils, encompassing 99 fibrils per region and depth per eye, were analyzed. Median collagen fibril diameter was 79.8 nm and ranged from 23 nm to 205 nm. This stereological approach provides a foundation for future studies investigating myopic scleral remodeling, where axial elongation and scleral thinning are known to show regional differences.

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来源期刊

Vision Research 医学-神经科学

CiteScore

3.70

自引率

16.70%

发文量

111

审稿时长

66 days

期刊介绍： Vision Research is a journal devoted to the functional aspects of human, vertebrate and invertebrate vision and publishes experimental and observational studies, reviews, and theoretical and computational analyses. Vision Research also publishes clinical studies relevant to normal visual function and basic research relevant to visual dysfunction or its clinical investigation. Functional aspects of vision is interpreted broadly, ranging from molecular and cellular function to perception and behavior. Detailed descriptions are encouraged but enough introductory background should be included for non-specialists. Theoretical and computational papers should give a sense of order to the facts or point to new verifiable observations. Papers dealing with questions in the history of vision science should stress the development of ideas in the field.