A validated liquid chromatography-tandem mass spectrometry assay for simultaneous quantitation of intact IGF-1 and IGF-2 in human serum

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B Pub Date : 2025-03-22 DOI:10.1016/j.jchromb.2025.124572

Hao-Long Zeng , Jie Lu , Huijun Li , Liming Cheng

{"title":"A validated liquid chromatography-tandem mass spectrometry assay for simultaneous quantitation of intact IGF-1 and IGF-2 in human serum","authors":"Hao-Long Zeng , Jie Lu , Huijun Li , Liming Cheng","doi":"10.1016/j.jchromb.2025.124572","DOIUrl":null,"url":null,"abstract":"<div><div>The simultaneous quantification of insulin-like growth factor (IGF) 1 and 2 has demonstrated significant potential for routine diagnostic applications in growth disorders. However, a simplified and rapid assay still remains to be developed. In this study, we established a simple, cost-effective and antibody-free serological assay based on tandem mass spectrometry to simultaneously quantify intact IGF-1 and IGF-2 in human serum. The lower limits of quantification were estimated to be 18.16 ng/mL for IGF-1 and 23.71 ng/mL for IGF-2. The linearity correlation coefficients exceeded 0.99 for both analytes. No significant matrix effects or carryover were observed. Intra- and inter-assay precisions were both less than 15 %. Recoveries from the standard addition assay fell within the range of 80 % to 120 %. We subsequently assessed the serum levels of IGF-1 and IGF-2 in a population residing in Wuhan, China, and found IGF-1 levels exhibited an age-related increase, peaking between 10 and 20 years of age, whereas IGF-2 levels remained consistently elevated across all age groups, although a relative decrease was noted between the ages of 20 and 30 years. This assay provides a simple, rapid, and immunoaffinity-free approach, rendering it more suitable for clinical applications aimed at assessing serum IGF-1 and IGF-2 levels.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1257 ","pages":"Article 124572"},"PeriodicalIF":2.8000,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Chromatography B","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1570023225001242","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

The simultaneous quantification of insulin-like growth factor (IGF) 1 and 2 has demonstrated significant potential for routine diagnostic applications in growth disorders. However, a simplified and rapid assay still remains to be developed. In this study, we established a simple, cost-effective and antibody-free serological assay based on tandem mass spectrometry to simultaneously quantify intact IGF-1 and IGF-2 in human serum. The lower limits of quantification were estimated to be 18.16 ng/mL for IGF-1 and 23.71 ng/mL for IGF-2. The linearity correlation coefficients exceeded 0.99 for both analytes. No significant matrix effects or carryover were observed. Intra- and inter-assay precisions were both less than 15 %. Recoveries from the standard addition assay fell within the range of 80 % to 120 %. We subsequently assessed the serum levels of IGF-1 and IGF-2 in a population residing in Wuhan, China, and found IGF-1 levels exhibited an age-related increase, peaking between 10 and 20 years of age, whereas IGF-2 levels remained consistently elevated across all age groups, although a relative decrease was noted between the ages of 20 and 30 years. This assay provides a simple, rapid, and immunoaffinity-free approach, rendering it more suitable for clinical applications aimed at assessing serum IGF-1 and IGF-2 levels.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Chromatography B 医学-分析化学

CiteScore

5.60

自引率

3.30%

发文量

306

审稿时长

44 days

期刊介绍： The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis. Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches. Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.