Misidentification of Raoultella spp. (R. terrigena, R. planticola) and Klebsiella spp. (K. variicola, K. grimontii) as Klebsiella pneumoniae: Retrospective study of a necropsy-associated bacterial collection from horses

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology Pub Date : 2025-03-28 DOI:10.1016/j.vetmic.2025.110497

Francois Gravey , Corinne Sévin , Bénédicte Langlois , Karine Maillard , Nathalie Foucher , Fabien Duquesne , Albertine Léon , Simon Le Hello , Sandrine Petry

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引用次数: 0

Abstract

Misidentifications as Klebsiella pneumoniae were observed during a French retrospective study of a necropsy-associated K. pneumoniae bacterial collection from horses. Accordingly, the present study aimed to further characterise the 12 Raoultella spp. and Klebsiella spp. strains involved in these misidentifications. The strains were identified and characterised using the Api 20E system, K. pneumoniae PCR detection, matrix-assisted laser desorption/ionisation time-of-flight mass spectroscopy and whole-genome sequencing. Antimicrobial susceptibilities were tested by the disc diffusion method with a panel of 40 antibiotics. Thus, misidentifications as K. pneumoniae mainly concerned Raoultella spp. (R. terrigena, R. planticola) rather than Klebsiella spp. (K. variicola, K. grimontii), with a dominance of R. terrigena. Among the 12 strains, only K. grimontii was multi-drug resistant and none were considered hypervirulent. MALDI-TOF was sufficient to avoid misidentification but commercial spectra databases should be expanded with K. grimontii and R. terrigena reference spectra to improve identification accuracy. This is probably (i) the first report of K. grimontii and R. planticola isolations from horses and (ii) the second report of R. terrigena and K. variicola isolations from horses.

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来源期刊

Veterinary microbiology 农林科学-兽医学

CiteScore

5.90

自引率

6.10%

发文量

221

审稿时长

52 days

期刊介绍： Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal. Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge. Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.