Propolis nanoemulsion extract from celebes stingless bee (Tetragonula biroi) phytochemistry and antibacterial analysis to periodontopathogen bacteria

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2025-03-28 DOI:10.1016/j.jobcr.2025.03.013

Nadya Rafika Amalia , Theresia Indah Budhy , Rini Devijanti Ridwan , Devi Rianti , Taufan Bramantoro , Muhammad Luthfi , Nastiti Faradilla Ramadhani , Adya Pramusita , Nurul Aisyah Rizki Putranti , Albertus Putera Nugraha , Putri Cahaya Situmorang , Khairul Anuar Shariff , Alexander Patera Nugraha

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引用次数: 0

Abstract

Background

Propolis from Sulawesi's stingless bees (Tetragonula biroi) contains antioxidants, more flavonoids than propolis from Apis bees, and the antibacterial ability.

Objective

to examine the antibacterial properties of Propolis Nanoemulsion Extract (PNE), which is extracted from the Celebes Stingless Bee (T. biroi), in relation to the periodontopathogen bacteria such as Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans (Aa), and Provotella intermedia (Pi). This investigation also examines PNE's phytochemistry, particle size analysis (PSA), and zeta potential.

Methods

The maceration process with 96 % ethanol was used to create PNE from Celebes stingless bee (T. biroi), which was then subjected to zeta potential measurement and PSA. Phytochemical analysis was used to identify phytochemical constituents in the PNE (T. biroi). Diffusion zone, minimum bactericidal concentration (MBC), and minimum inhibitory concentration (MIC) were used to assess antibacterial efficacy against Aa, Pg, Pi, and Fn. Furthermore, the statistical analysis was used to extract the data.

Results

Phenols, alkaloids, and flavonoids were identified; however, triterpenoids and saponins were not. Between 151.28 and 182.2 diameter nanometers (d.nm) was the range of the PNE's diameter. At 1.56 % propolis (T. biroi) concentration, the MIC, MBC, and diffusion zone analysis performed better than at 0.76 %, with a significant difference (p:0.01; p < 0.05) to Aa, Pg, Pi, and Fn.

Conclusions

The highest antibacterial activity against Aa, Pg, Pi, and Fn as periodontopathogen bacteria is demonstrated by alkaloids, flavonoids, and phenols in PNE from Celebes (T. biroi) at a concentration of 1.56 %.

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来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.