Using corn cob powder to induce AFB1-degrading enzyme production in Cladosporium uredinicola HSK8 for detoxification purposes

IF 2.6 4区医学 Q2 PHARMACOLOGY & PHARMACY

Toxicon Pub Date : 2025-03-25 DOI:10.1016/j.toxicon.2025.108333

Mengxiong Xiang , Ernuo Tian , Shuting Wang , Siyao Wang , Runmei Zhang , Jianyi Guo , Ming Liu , Qiang Ding , Jun Cai

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引用次数: 0

Abstract

Aflatoxin B₁ (AFB₁) is a highly toxic mycotoxin that contaminates food sources and poses serious health risks. This study examines Cladosporium uredinicola HSK8's ability to degrade AFB₁. It was demonstrated that the hydrolysis of corn cob powder, following high-temperature treatment, generates derivatives such as furfural and vanillin, which stimulate the production of various AFB₁-degrading enzymes by C. uredinicola HSK8. The total enzymatic activity reached a peak of 3750 U/mL in fermentation broth with 3 % corn cob powder. Two AFB₁-degrading enzymes were further isolated, purified and analyzed. The results showed that enzymes can disrupt both the furan and benzene rings, exhibiting strong catalytic activity. The results suggested corn cob powder as a complex carbon source not only promotes the growth of C. uredinicola but also induces the production of AFB₁-degrading enzymes. Therefore, the fermentation broth of C. uredinicola HSK8 cultured with corn cob powder holds great potential for the detoxification of AFB₁ in food and feed.

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来源期刊

Toxicon 医学-毒理学

CiteScore

4.80

自引率

10.70%

发文量

358

审稿时长

68 days

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