Mechanobiological reprogramming in liver cancer: Conjugated linoleic acid disrupts EMT and cytoskeletal dynamics

IF 4.9 2区化学 Q2 CHEMISTRY, PHYSICAL

Colloids and Surfaces A: Physicochemical and Engineering Aspects Pub Date : 2025-03-28 DOI:10.1016/j.colsurfa.2025.136744

Ibrahim Ghoytasi , Mohammad Kazemi Ashtiani , Mahsa Ghasemzad , Abbas Piryaei , Siavash Iravani , Ali Zarrabi , Massoud Vosough

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引用次数: 0

Abstract

The excessive increase in hepatocellular carcinoma (HCC) incidence and poor therapeutic responses have led researchers to focus on innovative approaches like differentiation therapy. An anticancer agent, conjugated linoleic acid (CLA), can induce differentiation and inhibit epithelial-mesenchymal transition (EMT) in HCC cells through the upregulation of hepatocyte nuclear factor 4 alpha (HNF4α). This study aims to investigate the mechanobiological alterations, which are important in metastasis, in HCC cells after CLA treatment. Hep3B cells were treated with different concentrations of CLA. The morphology, viability, migration ability, and variation in the density and localization of the cell’s cytoskeleton filaments (CSFs) were evaluated. Then, the induction of epithelial morphology in the cells was quantified using different geometric criteria. Finally, the effect of CLA treatment on the elastic and viscoelastic properties of the cells was investigated. CLA treatment suppressed the individual and collective migration of HCC cells and reduced their dynamic movements. With increasing concentrations of CLA, the ratio of the relative fluorescent of the filaments (RFF) decreased in the nucleus and increased in cytoplasm areas. However, the expression of vimentin in all parts of the cells was remarkably reduced. Additionally, CLA treatment increased the epithelial characteristics of the HCC cells from the morphological perspective and improved their viscoelastic properties. CLA treatment reduced the cancerous characteristics of HCC cells and inhibited the EMT process by strengthening the mechanobiological features and rearranging their cytoskeleton network.

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来源期刊

Colloids and Surfaces A: Physicochemical and Engineering Aspects 化学-物理化学

CiteScore

8.70

自引率

9.60%

发文量

2421

审稿时长

56 days

期刊介绍： Colloids and Surfaces A: Physicochemical and Engineering Aspects is an international journal devoted to the science underlying applications of colloids and interfacial phenomena. The journal aims at publishing high quality research papers featuring new materials or new insights into the role of colloid and interface science in (for example) food, energy, minerals processing, pharmaceuticals or the environment.