Blood and colostral IgM and IgG B cell repertoires in high, average, and low immune responder Holstein Friesian cows and heifers

IF 1.4 3区农林科学 Q4 IMMUNOLOGY

Veterinary immunology and immunopathology Pub Date : 2025-03-28 DOI:10.1016/j.vetimm.2025.110926

T.E. Altvater-Hughes , H.P. Hodgins , D.C. Hodgins , C.A. Bauman , B.A. Mallard

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引用次数: 0

Abstract

In dairy cattle, genetic selection for higher antibody-mediated (AMIR) and cell-mediated (CMIR) immune responses can enhance disease resistance. Cattle produce a unique subset of B cells with B cell receptors with ultralong complementarity determining regions 3 (CDR3). Antibodies with these specialized structures have superior virus neutralization characteristics. Published studies of B cell receptors with ultralong CDR3s in dairy cattle have been limited by the number of animals examined (1–4 animals in each study), and by varying breeds and ages. The objective of this study was to assess the percentage of IgM and IgG sequences with ultralong CDR3s, and gene usage in blood and colostral lymphocytes from cows classified as high, average, and low immune responders based on their estimated breeding values. B lymphocytes were isolated from the blood of 14 heifers and 7 cows. In addition, cells were isolated from colostrum of the 7 cows. RNA was extracted, cDNA was produced, and IgM and IgG transcripts were amplified using polymerase chain reactions. Amplicons were sequenced using Oxford Nanopore long-read sequencing. In sequences derived from blood B cells, AMIR estimated breeding values were significantly and positively associated with higher percentages of IgG ultralong CDR3 sequences. High AMIR cows (n = 3) also produced colostrum with a significantly greater percentage of IgG ultralong CDR3 sequences (18.0 %) than average AMIR cows (n = 4, mean 8.8 %). Larger studies are needed to investigate the association between percentages of B cells expressing IgG ultralong CDR3s and observed health traits.

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来源期刊

Veterinary immunology and immunopathology 农林科学-免疫学

CiteScore

3.40

自引率

5.60%

发文量

审稿时长

70 days

期刊介绍： The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.