The effect of ultrasonic-assisted enzymes extraction on antioxidant polysaccharide activity in dandelion

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry Pub Date : 2025-03-28 DOI:10.1016/j.ultsonch.2025.107329

Shuang Ni , Haolan Zhao , Shaohua Yang , Kai Cui

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Abstract

In this study, ultrasonic-assisted enzymatic extraction (UAEE) method was used to obtain the small molecule dandelion polysaccharides. Based on single-factor experiments, the process of extraction the extraction conditions were further optimized by response surface method (RSM) combined with the Box-Behnken design (BBD). The results showed that in combination with enzymes, with a ratio of 1:32 (g/ml), an enzymolysis temperature 55 °C, an ultrasonic temperature 75℃ and the ultrasonic time 55 min. The maximum extraction yield of crude polysaccharides was 3.127 %. After purification by DEAE-cellulose chromatography and Sephadex G-75 gel filtration, a novel polysaccharide (DANP-Ⅱ) was extracted and the results of high performance liquid chromatography (HPLC) indicated its average molecular weight was 3.373 kDa. Gas chromatography (GC) analysis showed that DANP-Ⅱ was mainly contained by glucose and little of glucuronic acid, mannose, galactose, and arabinose. In vitro antioxidant experiments have demonstrated that DANP-Ⅱ can alleviate H2O2-induced cellular damage, reduce apoptosis rates, and exhibit robust antioxidant activity. Hence, DANP-Ⅱ can be utilized as a natural antioxidant to enhance protection against oxidative stress, providing theoretical guidance for the utilization of low-molecular-weight dandelion polysaccharides.

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超声波辅助酶提取对蒲公英抗氧化多糖活性的影响

本研究采用超声辅助酶法提取蒲公英小分子多糖。在单因素试验的基础上，采用响应面法（RSM）结合Box-Behnken设计（BBD）对提取工艺进行进一步优化。结果表明，在酶解比为1:32 （g/ml）、酶解温度为55℃、超声温度为75℃、超声时间为55 min的条件下，粗多糖提取率可达3.127%。经deae -纤维素层析和Sephadex G-75凝胶过滤纯化后，提取到一种新的多糖（DANP-Ⅱ），高效液相色谱（HPLC）结果表明其平均分子量为3.373 kDa。气相色谱（GC）分析表明，DANP-Ⅱ主要由葡萄糖组成，葡萄糖醛酸、甘露糖、半乳糖和阿拉伯糖含量较少。体外抗氧化实验表明，DANP-Ⅱ可以减轻h2o2诱导的细胞损伤，降低细胞凋亡率，并表现出强大的抗氧化活性。因此，DANP-Ⅱ可以作为一种天然抗氧化剂，增强对氧化应激的保护作用，为低分子量蒲公英多糖的利用提供理论指导。

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来源期刊

Ultrasonics Sonochemistry 化学-化学综合

CiteScore

15.80

自引率

11.90%

发文量

361

审稿时长

59 days

期刊介绍： Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.