Depressive-Like Behavior and Liver Damage Generate Behavioral and Cortical Microglial Morphological Differences in Mice.

Current health sciences journal Pub Date : 2024-10-01 Epub Date: 2024-12-31 DOI:10.12865/CHSJ.50.04.08
Gabriel Nedelea, Mădălina Iuliana Mușat, Alina Cătălina Buican-Chirea, Mihai Călin Ciorbagiu, Bogdan Cătălin
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Abstract

Introduction: The correlation between nonalcoholic fatty liver disease (NAFLD) and depression has already been established, but the relation between the two is insufficiently studied. Various murine models have proven effective in evaluating the mechanisms involved in these pathologies.

Material and methods: In this study we aimed to assess how the chronic unpredictable mild stress (CUMS) protocol impacts the behavior of mice with liver damage induced by a methionine choline deficient (MCD) diet, and also to evaluate the changes in microglial morphology in the cortex of animals with depressive-like behavior and liver injury. Thus, the cortical region was analyzed using confocal microscopy.

Results: Sucrose preference test and open field test revealed induced anhedonia and anxiety-like behavior, but short-term memory was not impaired, as assessed by novel object recognition test. Administration of the MCD diet led to an increase in total branch length and the number of terminal branches, revealing a hyperactivated microglia. The CUMS protocol in combination with MCD diet induced a reduced branching complexity, with fewer tertiary and terminal branches.

Conclusion: Our study highlights the importance of microglial morphology at the cortical level in coexisting depression and liver injury.

抑郁样行为和肝损伤对小鼠行为和皮质小胶质细胞形态学的影响。
简介:非酒精性脂肪性肝病(NAFLD)与抑郁症之间的相关性已经确立,但两者之间的关系研究不足。各种小鼠模型已被证明在评估涉及这些病理机制方面是有效的。材料和方法:在本研究中,我们旨在评估慢性不可预测轻度应激(CUMS)方案如何影响蛋氨酸胆碱缺乏(MCD)饮食诱导的肝损伤小鼠的行为,并评估抑郁样行为和肝损伤动物皮质小胶质细胞形态的变化。因此,使用共聚焦显微镜分析皮质区域。结果:蔗糖偏好测试和开放场地测试显示诱导的快感缺乏和焦虑样行为,但新物体识别测试评估短期记忆未受损。MCD饮食导致总分支长度和末端分支数量的增加,显示出过度激活的小胶质细胞。CUMS方案结合MCD饮食诱导分支复杂性降低,三级和末端分支减少。结论:我们的研究强调了皮层水平的小胶质细胞形态在并存的抑郁症和肝损伤中的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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