In vitro and in vivo protective effects of a phytochemical mixture (Posoqueria latifolia extract + Kaempferol) against UVB-induced radiation damage.

IF 2.6 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Silvia Ximena Barrios Martínez, Diego Armando Villamizar Mantilla, Nathaly Hernández-Díaz, Lady Johanna Sierra, Raquel Elvira Ocazionez, Elena E Stashenko, María Pilar Vinardell, Jorge Luis Fuentes
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引用次数: 0

Abstract

Plants may be a source of protective compounds that inhibit injuries caused by overexposure to solar radiation. Present work evaluated the protective effects of a phytochemical mixture based on Posoqueria latifolia extract + Kaempferol against ultraviolet-induced radiation DNA damage in human fibroblasts and mice skin. Cytotoxicity, genotoxicity, and antigenotoxicity of P. latifolia flower extract, Kaempferol, and the mixture of both were studied in MRC5 human fibroblasts using Trypan blue exclusion and Comet assays. Kinetics of DNA damage repair post irradiation, in the presence and absence of the phytochemicals, were also evaluated. The inhibition of UVB-induced erythema and cyclobutene pyrimidine dimers (CPD) by the phytochemicals and the mixture were also investigated in mice skin. We showed that the P. latifolia flower extract, Kaempferol, and the mixture inhibited UVB-induced DNA damage and increased DNA repair mechanisms in human fibroblasts. In addition, these phytochemicals inhibited the UVB-induced erythema and CPD in mice skin. The potential of the studied phytochemicals in human photoprotection was discussed.

植物化学混合物(荷叶提取物+山奈酚)对uvb诱导的辐射损伤的体内外保护作用
植物可能是一种保护性化合物的来源,这种化合物可以抑制因过度暴露于太阳辐射而造成的伤害。本研究评价了以荷叶提取物+山奈酚为基础的植物化学混合物对紫外线诱导的人成纤维细胞和小鼠皮肤DNA损伤的保护作用。采用台斑蓝排斥法和Comet法,研究了香樟花提取物、山奈酚及其混合物在MRC5人成纤维细胞中的细胞毒性、遗传毒性和抗遗传毒性。在存在和不存在植物化学物质的情况下,辐照后DNA损伤修复动力学也进行了评估。研究了植物化学物质及其混合物对uvb诱导的小鼠皮肤红斑和环丁烯嘧啶二聚体(CPD)的抑制作用。我们发现,油葵花提取物、山奈酚和混合物抑制了uvb诱导的DNA损伤,并增加了人成纤维细胞的DNA修复机制。此外,这些植物化学物质还能抑制uvb诱导的小鼠皮肤红斑和CPD。讨论了所研究的植物化学物质在人体光防护中的潜力。
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来源期刊
Photochemistry and Photobiology
Photochemistry and Photobiology 生物-生化与分子生物学
CiteScore
6.70
自引率
12.10%
发文量
171
审稿时长
2.7 months
期刊介绍: Photochemistry and Photobiology publishes original research articles and reviews on current topics in photoscience. Topics span from the primary interaction of light with molecules, cells, and tissue to the subsequent biological responses, representing disciplinary and interdisciplinary research in the fields of chemistry, physics, biology, and medicine. Photochemistry and Photobiology is the official journal of the American Society for Photobiology.
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