Effect of photodynamic therapy on the expression of toll-like receptor 2, -3, -4, and -8 in the cervical lesions.

IF 2.6 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Alexander Dushkin, Afanasiev Maxim, Svitich Oxana, Afanasiev Stanislav, Grishacheva Tatyana, Kukina Polina, Asmik Avagyan, Irina Dushkina, Elena Biryukova, Akmaral Khangeldi, Alexander Karaulov
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Abstract

Photodynamic therapy (PDT) is a minimally invasive treatment with low systemic toxicity and immunomodulatory effects, increasingly applied in managing HPV-associated cervical lesions. Toll-like receptors (TLRs) are critical in regulating immune responses in cervical pathology, yet their dynamics under PDT remain underexplored. This study investigates the effect of PDT on TLR2, TLR3, TLR4, and TLR8 expression in cervical epithelial cells ex vivo, considering lesion severity. The results reveal that TLR8 exhibited the most significant reduction across all groups 2 h after PDT, with the strongest suppression in patients with invasive cervical cancer. TLR4 expression decreased by 24% in HPV-infected patients and by 71% in those with cervical cancer, highlighting its potential role in modulating the inflammatory microenvironment post-PDT. TLR3 showed hyperexpression in LSIL and suppression in HSIL/CIN III, although changes were not statistically significant (p > 0.05). TLR2 expression remained unchanged, likely due to HPV type variability. These findings demonstrate that PDT effectively reduces hyperexpression of TLR8, TLR4, and TLR3 in early-stage cervical cancer, suggesting their potential as biomarkers for treatment efficacy. Further research incorporating HPV typing and advanced techniques like liquid biopsy is essential to refine our understanding of TLRs in PDT for HPV-associated cervical lesions.

光动力治疗对宫颈病变中toll样受体2、-3、-4和-8表达的影响。
光动力疗法(PDT)是一种具有低全身毒性和免疫调节作用的微创治疗方法,越来越多地应用于hpv相关宫颈病变的治疗。toll样受体(TLRs)在调节宫颈病理中的免疫反应中起着至关重要的作用,但其在PDT下的动态仍未得到充分研究。本研究考虑病变严重程度,探讨PDT对离体宫颈上皮细胞TLR2、TLR3、TLR4和TLR8表达的影响。结果显示,在PDT后2小时,TLR8在所有组中表现出最显著的降低,其中浸润性宫颈癌患者的抑制最强。TLR4表达在hpv感染患者中下降24%,在宫颈癌患者中下降71%,突出了其在pdt后炎症微环境调节中的潜在作用。TLR3在LSIL中高表达,在HSIL/CIN III中抑制,但变化无统计学意义(p < 0.05)。TLR2的表达保持不变,可能是由于HPV类型的变异性。这些发现表明,PDT可以有效降低TLR8、TLR4和TLR3在早期宫颈癌中的高表达,提示它们有可能作为治疗效果的生物标志物。结合HPV分型和液体活检等先进技术的进一步研究对于完善我们对HPV相关宫颈病变PDT中tlr的理解至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Photochemistry and Photobiology
Photochemistry and Photobiology 生物-生化与分子生物学
CiteScore
6.70
自引率
12.10%
发文量
171
审稿时长
2.7 months
期刊介绍: Photochemistry and Photobiology publishes original research articles and reviews on current topics in photoscience. Topics span from the primary interaction of light with molecules, cells, and tissue to the subsequent biological responses, representing disciplinary and interdisciplinary research in the fields of chemistry, physics, biology, and medicine. Photochemistry and Photobiology is the official journal of the American Society for Photobiology.
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