Functional Genomics Using High-Throughput Methods for Salivary Analysis in Sjögren's Disease.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2025-03-27 DOI:10.1111/odi.15328

Giovanna P Florezi, Mônica Teresa Veneziano Labate, Carlos Alberto Labate, Felippe P Barone, Silvia Vanessa Lourenço

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引用次数: 0

Abstract

Objectives: Primary Sjögren's disease (SjD) is a slow-progressing autoimmune disease that affects salivary and lacrimal glands, causing dry eyes (xerophthalmia) and dry mouth (xerostomia). Diagnosing SjD involves clinical, serological, and histological assessments, but reliable biomarkers are lacking.

Methods: This study analyzed the metabolic and proteomic profiles of 19 female SjD patients (based on ACR-EULAR criteria) compared to 20 healthy individuals. Saliva and blood samples were analyzed using mass spectrometry and chromatography. Proteomic analysis was performed with the nanoElute nanoflow system coupled to a timsTof-Pro mass spectrometer, and metabolic profiling with a GC-TOF/MS Pegasus HT.

Results: Statistical tests identified significant differences in metabolites and proteins between SjD patients and controls. Metabolome analysis revealed changes in amino acid synthesis, purine and lipid metabolism, and exposure to external compounds. Proteomic analysis indicated immune-related proteins and inflammatory lipid metabolism. GNAI2, B2MG, NGAL, SLUR2, HS90, SODC, and A2GL emerged as potential biomarkers for SjD.

Conclusion: This study demonstrates the potential of high-performance techniques in identifying biomarkers for SjD diagnosis and prognosis. Data are available via ProteomeXchange (PXD055629).

查看原文本刊更多论文

使用高通量方法进行Sjögren病唾液分析的功能基因组学

目的：原发性Sjögren病（SjD）是一种进展缓慢的自身免疫性疾病，影响唾液腺和泪腺，引起眼干（干眼症）和口干（口干症）。SjD的诊断涉及临床、血清学和组织学评估，但缺乏可靠的生物标志物。方法：本研究分析了19名女性SjD患者（基于ACR-EULAR标准）与20名健康个体的代谢和蛋白质组学特征。唾液和血液样本用质谱和色谱分析。蛋白质组学分析使用nanoElute纳米流系统与timstop - pro质谱联用，代谢谱分析使用GC-TOF/MS Pegasus HT。结果：统计测试发现SjD患者和对照组之间的代谢物和蛋白质有显著差异。代谢组学分析揭示了氨基酸合成、嘌呤和脂质代谢以及暴露于外部化合物的变化。蛋白质组学分析显示免疫相关蛋白和炎症脂质代谢。GNAI2、B2MG、NGAL、SLUR2、HS90、SODC和A2GL被认为是SjD的潜在生物标志物。结论：本研究显示了在SjD诊断和预后中识别生物标志物的高性能技术的潜力。数据可通过ProteomeXchange （PXD055629）获得。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.