Development of Layer-by-Layer Magnetic Nanoparticles for Application to Radiotherapy of Pancreatic Cancer.

IF 4.2 2区 化学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Nobuyoshi Fukumitsu, Yoshitaka Matsumoto, Lili Chen, Yu Sugawara, Nanami Fujisawa, Eri Niiyama, Sosuke Ouchi, Emiho Oe, Takashi Saito, Mitsuhiro Ebara
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引用次数: 0

Abstract

Pancreatic cancer is among the deadliest malignancies, with few treatment options for locally advanced, unresectable cases. Conventional therapies, such as chemoradiotherapy and hyperthermia, show promise but face challenges in improving outcomes. This study introduces a novel drug delivery system using gemcitabine (GEM)-loaded layer-by-layer magnetic nanoparticles (LBL MNPs) combined with alternating magnetic field (AMF) application and X-ray irradiation to enhance therapeutic efficacy. LBL MNPs were synthesized using optimized layering techniques to achieve superior drug loading and controlled release. Human pancreatic cancer cells (PANC-1) were treated with LBL MNPs alone, with AMF-induced hyperthermia, and in combination with X-rays. The results demonstrate that the 7-layer LBL MNPs exhibited optimal cytotoxicity, significantly reducing cell viability at concentrations of 30 µg/mL and higher. Combining 7-layer LBL MNPs with AMF increased cell death in a time- and concentration-dependent manner, achieving up to 98% inhibition of cell proliferation. The addition of X-rays to the regimen demonstrated a strong synergistic effect, resulting in a 13-fold increase in cell death compared to controls. These findings highlight the potential of this integrated approach to improve outcomes in patients with pancreatic cancer.

层状磁性纳米颗粒在胰腺癌放射治疗中的应用。
胰腺癌是最致命的恶性肿瘤之一,对于局部晚期、不可切除的病例,几乎没有治疗选择。传统疗法,如放化疗和热疗,显示出希望,但在改善结果方面面临挑战。本研究介绍了一种新型的药物递送系统,利用吉西他滨(GEM)负载的层层磁性纳米颗粒(LBL MNPs)结合交变磁场(AMF)应用和x射线照射来提高治疗效果。采用优化的分层技术合成了LBL MNPs,以达到良好的载药量和控释效果。人胰腺癌细胞(PANC-1)分别用LBL MNPs、amf诱导的热疗和x射线联合治疗。结果表明,7层LBL MNPs表现出最佳的细胞毒性,在浓度为30µg/mL或更高时显著降低细胞活力。将7层LBL MNPs与AMF结合后,细胞死亡率呈时间和浓度依赖性增加,细胞增殖抑制率高达98%。在治疗方案中加入x射线显示出强大的协同效应,与对照组相比,导致细胞死亡增加13倍。这些发现强调了这种综合方法改善胰腺癌患者预后的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Molecules
Molecules 化学-有机化学
CiteScore
7.40
自引率
8.70%
发文量
7524
审稿时长
1.4 months
期刊介绍: Molecules (ISSN 1420-3049, CODEN: MOLEFW) is an open access journal of synthetic organic chemistry and natural product chemistry. All articles are peer-reviewed and published continously upon acceptance. Molecules is published by MDPI, Basel, Switzerland. Our aim is to encourage chemists to publish as much as possible their experimental detail, particularly synthetic procedures and characterization information. There is no restriction on the length of the experimental section. In addition, availability of compound samples is published and considered as important information. Authors are encouraged to register or deposit their chemical samples through the non-profit international organization Molecular Diversity Preservation International (MDPI). Molecules has been launched in 1996 to preserve and exploit molecular diversity of both, chemical information and chemical substances.
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