Red Mud Potentially Alleviates Ammonia Nitrogen Inhibition in Swine Manure Anaerobic Digestion by Enhancing Phage-Mediated Ammonia Assimilation.

IF 4.1 2区 生物学 Q2 MICROBIOLOGY
Yulong Peng, Luhua Jiang, Junzhao Wu, Jiejie Yang, Ziwen Guo, Manjun Miao, Zhiyuan Peng, Meng Chang, Bo Miao, Hongwei Liu, Yili Liang, Huaqun Yin, Qiang He, Xueduan Liu
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Abstract

Red mud has been demonstrated to improve the methane production performance of anaerobic digestion (AD). However, the influence of red mud on ammonia nitrogen inhibition during AD through the mediating role of bacteria-phages interactions in this process remains poorly understood. Thus, this study investigated the impact of red mud on nitrogen metabolism in AD and characterized the phage and prokaryotic communities through a metagenomic analysis. The results showed that red mud significantly increased methane production by 23.1% and promoted the conversion of ammonia nitrogen into organic nitrogen, resulting in a 4.8% increase in total nitrogen. Simultaneously, it enriched the key microbial genera Methanothrix, Proteinophilum, and Petrimonas by 0.5%, 0.8%, and 2.7%, respectively, suggesting an enhancement in syntrophic acetate oxidation with greater ammonia tolerance. A viral metagenomic analysis identified seven nitrogen-metabolism-related auxiliary metabolic genes (AMGs), with glnA (encoding glutamine synthetase) being the most abundant. Compared to the control treatments, the red mud treatments led to a higher abundance of temperate phages and an increased number of AMGs. Furthermore, two new hosts carrying glnA (Mycolicibacteria smegmatis and Kitasatopola aureofaciens) were predicted, indicating that red mud expanded the host range of phages and promoted the spread of AMGs. Overall, our findings highlight the importance of phages in alleviating ammonia nitrogen inhibition and provide a novel understanding of the role of red mud in the AD of swine manure.

赤泥可能通过增强噬菌体介导的氨同化来缓解猪粪厌氧消化中氨氮的抑制。
赤泥已被证明可以提高厌氧消化(AD)的产甲烷性能。然而,赤泥通过介导细菌-噬菌体相互作用对AD过程中氨氮抑制的影响尚不清楚。因此,本研究研究了赤泥对AD中氮代谢的影响,并通过宏基因组分析对噬菌体和原核生物群落进行了表征。结果表明,赤泥显著提高了甲烷产量23.1%,促进了氨氮向有机氮的转化,总氮增加4.8%。同时,主要微生物属Methanothrix、Proteinophilum和Petrimonas的含量分别增加了0.5%、0.8%和2.7%,表明合成氨乙酸氧化能力增强,耐氨能力增强。一项病毒宏基因组分析发现了7个与氮代谢相关的辅助代谢基因(AMGs),其中glnA(编码谷氨酰胺合成酶)含量最多。与对照处理相比,赤泥处理导致温带噬菌体丰度更高,AMGs数量增加。此外,还预测了两种携带glnA的新宿主(耻垢分枝杆菌和金黄色北孢菌),表明赤泥扩大了噬菌体的宿主范围,促进了AMGs的传播。总之,我们的研究结果强调了噬菌体在缓解氨氮抑制中的重要性,并为赤泥在猪粪AD中的作用提供了新的认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Microorganisms
Microorganisms Medicine-Microbiology (medical)
CiteScore
7.40
自引率
6.70%
发文量
2168
审稿时长
20.03 days
期刊介绍: Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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