Isolation and Identification of Bacterial Strains Colonizing the Surface of Biodegradable Polymers.

Abstract

Plastics play a key role in every sector of the economy, being used in the manufacturing of products in the fields of health, food packaging, and agriculture. Their mismanagement poses a serious threat to ecosystems and, in general, to human life. For this reason, particular attention has been paid in the last decade to the use of biodegradable polymers (BPs) as an alternative to classic plastics. In this study, we aimed to identify bacterial strains able to colonize the surface of five BPs: poly(butylene succinate) (PBS), poly(butylene succinate-co-butylene adipate) (PBSA), poly(ε-caprolactone), (PCL), poly(3-hydroxybutyrate) (PHB), and poly(lactic acid) (PLA). For this experiment, mesocosms were designed ad hoc to mimic the conditions in which the polymers can be found in marine environments: i. suspended in the water column; ii. laying over gravel; and iii. under gravel. Four bacterial samples were taken (3, 4, 10, and 12 months from the start of the experiment) from five BPs incubated in the above-mentioned three conditions. Our results demonstrated that bacteria belonging to the Proteobacteria, Actinobacteria, Firmicutes, Bacillota, Bacteroidota, and Cyanobacteria phyla were the most frequent colonizers of the surfaces of the five polymers under analysis, and could be responsible for their degradation, resulting in the evolution of strategies to degrade plastics through the secretion of specific enzymes.