In Infants with Neuroblastoma Standard Therapy Only Partially Reverts the Fecal Microbiome Dysbiosis Present at Diagnosis.

IF 4.1 2区 生物学 Q2 MICROBIOLOGY
Maria Valeria Corrias, Eddi Di Marco, Carola Bonaretti, Margherita Squillario, Loredana Amoroso, Massimo Conte, Mirco Ponzoni, Roberto Biassoni
{"title":"In Infants with Neuroblastoma Standard Therapy Only Partially Reverts the Fecal Microbiome Dysbiosis Present at Diagnosis.","authors":"Maria Valeria Corrias, Eddi Di Marco, Carola Bonaretti, Margherita Squillario, Loredana Amoroso, Massimo Conte, Mirco Ponzoni, Roberto Biassoni","doi":"10.3390/microorganisms13030691","DOIUrl":null,"url":null,"abstract":"<p><p>The fecal microbiomes of 15 infants with neuroblastoma (NB) at the onset of the disease and after standard-of-care therapy have been prospectively analyzed compared to those of age-matched healthy infants. By applying several algorithms to 16S sequencing, we found that the fecal microbiomes of infants with NB at onset were abundant in <i>Pseudomonadota</i>, including different descendants of <i>Gammaproteobacteria.</i> After completing therapy, their abundance decreased to a level like that observed in healthy infants. In contrast, the <i>Bacillota</i> that showed at the onset an abundance like that of healthy infants doubled their amount after treatment. In infants with NB, the beta diversity of the fecal microbiomes was significantly reduced compared to healthy infants and patients at the end of treatment. The Random Forest algorithm and the Reingold-Tilford heat tree showed that <i>Enterobacteriaceae</i> had a higher abundance at the onset, which declined after therapy. Picrust2 inferred pathway analysis indicated that the drug treatment was associated with a reduction in the polyamine pathway, highly represented in samples of NB at the onset. In conclusion, the dysbiosis observed in infants with NB at onset changed following standard-of-care treatment. Still, the composition at the end of treatment did not completely resemble that of healthy infants.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1000,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11946756/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microorganisms","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/microorganisms13030691","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

The fecal microbiomes of 15 infants with neuroblastoma (NB) at the onset of the disease and after standard-of-care therapy have been prospectively analyzed compared to those of age-matched healthy infants. By applying several algorithms to 16S sequencing, we found that the fecal microbiomes of infants with NB at onset were abundant in Pseudomonadota, including different descendants of Gammaproteobacteria. After completing therapy, their abundance decreased to a level like that observed in healthy infants. In contrast, the Bacillota that showed at the onset an abundance like that of healthy infants doubled their amount after treatment. In infants with NB, the beta diversity of the fecal microbiomes was significantly reduced compared to healthy infants and patients at the end of treatment. The Random Forest algorithm and the Reingold-Tilford heat tree showed that Enterobacteriaceae had a higher abundance at the onset, which declined after therapy. Picrust2 inferred pathway analysis indicated that the drug treatment was associated with a reduction in the polyamine pathway, highly represented in samples of NB at the onset. In conclusion, the dysbiosis observed in infants with NB at onset changed following standard-of-care treatment. Still, the composition at the end of treatment did not completely resemble that of healthy infants.

在患有神经母细胞瘤的婴儿中,标准治疗只能部分恢复诊断时存在的粪便微生物群失调。
与年龄匹配的健康婴儿相比,对15例神经母细胞瘤(NB)婴儿在发病时和经过标准护理治疗后的粪便微生物组进行了前瞻性分析。通过将几种算法应用于16S测序,我们发现新生儿NB发病时的粪便微生物组在假单胞菌中丰富,包括不同的γ变形菌门后代。在完成治疗后,它们的丰度下降到与健康婴儿相似的水平。相比之下,在开始时显示出与健康婴儿相同数量的芽孢杆菌在治疗后数量翻了一番。在NB婴儿中,与健康婴儿和治疗结束时的患者相比,粪便微生物组的β多样性显着降低。随机森林算法和Reingold-Tilford热树显示Enterobacteriaceae在发病时丰度较高,治疗后丰度下降。Picrust2推断途径分析表明,药物治疗与多胺途径的减少有关,在发病时NB样品中高度代表。综上所述,新生儿新生儿的生态失调在标准治疗后发生了改变。尽管如此,治疗结束时的成分与健康婴儿的成分并不完全相似。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Microorganisms
Microorganisms Medicine-Microbiology (medical)
CiteScore
7.40
自引率
6.70%
发文量
2168
审稿时长
20.03 days
期刊介绍: Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信