Mateusz Szymański, Małgorzata M. Skiba, Małgorzata Piasecka
{"title":"Determining the Usefulness of Selected Laboratory Markers of Inflammation in Qualifying Patients for T2 Biosystems Determination","authors":"Mateusz Szymański, Małgorzata M. Skiba, Małgorzata Piasecka","doi":"10.1002/jcla.70021","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Improving treatment outcomes sepsis requires early recognition, the implementation of appropriate treatment, and targeted antimicrobial therapy. Nowadays, microbiological diagnostic methods are available to accelerate microbiological diagnosis, thereby reducing the time needed to implement targeted antibiotic therapy. One method for rapid diagnosis is the amplified magnetic resonance imaging—T2 Biosystems, USA (T2Dx). This method enables the identification of pathogens directly from a blood sample (approximately 4 mL) within about 3.5 h. The use of the “T2 Resistance” panel additionally allows for the detection of the most common bacterial resistance mechanisms in about 4–5 h. The disadvantage of the T2Dx method is the limited number of microorganisms it can detect. The objective of the study was to evaluate the effectiveness of using selected inflammatory parameters to accurately qualify patients (positive result) for T2Dx testing.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>We have made a retrospective evaluation of selected inflammatory parameters in order to determine which parameters are the best indicators for good qualification of patients.</p>\n </section>\n \n <section>\n \n <h3> Results and Conclusion</h3>\n \n <p>A single analysis of parameters such as C-reactive protein (CRP), white blood cells (WBC), #neutr, #lymph, and neutrophil-to-lymphocyte ratio (NLR) is not a good indicator that could be used as an additional tool facilitating patient qualification for T2Dx testing. The most sensitive parameter distinguishing between patients with a positive T2Dx result and those with a negative result is the measurement of IL-6 and PCT. Proper patient qualification for T2Dx testing can significantly contribute to reducing the time to initiate targeted antibiotic therapy and may impact reducing mortality and improving long-term treatment outcomes.</p>\n </section>\n </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 8","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70021","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Clinical Laboratory Analysis","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jcla.70021","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background
Improving treatment outcomes sepsis requires early recognition, the implementation of appropriate treatment, and targeted antimicrobial therapy. Nowadays, microbiological diagnostic methods are available to accelerate microbiological diagnosis, thereby reducing the time needed to implement targeted antibiotic therapy. One method for rapid diagnosis is the amplified magnetic resonance imaging—T2 Biosystems, USA (T2Dx). This method enables the identification of pathogens directly from a blood sample (approximately 4 mL) within about 3.5 h. The use of the “T2 Resistance” panel additionally allows for the detection of the most common bacterial resistance mechanisms in about 4–5 h. The disadvantage of the T2Dx method is the limited number of microorganisms it can detect. The objective of the study was to evaluate the effectiveness of using selected inflammatory parameters to accurately qualify patients (positive result) for T2Dx testing.
Methods
We have made a retrospective evaluation of selected inflammatory parameters in order to determine which parameters are the best indicators for good qualification of patients.
Results and Conclusion
A single analysis of parameters such as C-reactive protein (CRP), white blood cells (WBC), #neutr, #lymph, and neutrophil-to-lymphocyte ratio (NLR) is not a good indicator that could be used as an additional tool facilitating patient qualification for T2Dx testing. The most sensitive parameter distinguishing between patients with a positive T2Dx result and those with a negative result is the measurement of IL-6 and PCT. Proper patient qualification for T2Dx testing can significantly contribute to reducing the time to initiate targeted antibiotic therapy and may impact reducing mortality and improving long-term treatment outcomes.
背景:提高脓毒症的治疗效果需要早期识别,实施适当的治疗,并有针对性地进行抗菌治疗。目前,微生物学诊断方法可以加速微生物学诊断,从而减少实施靶向抗生素治疗所需的时间。快速诊断的一种方法是放大磁共振成像- t2 Biosystems, USA (T2Dx)。该方法能够在3.5小时内直接从血液样本(约4ml)中鉴定病原体。“T2耐药”面板的使用还允许在约4-5小时内检测最常见的细菌耐药机制。T2Dx法的缺点是它能检测到的微生物数量有限。该研究的目的是评估使用选定的炎症参数来准确确定患者(阳性结果)进行T2Dx检测的有效性。方法:我们对选定的炎症参数进行回顾性评价,以确定哪些参数是最佳的指标,以获得良好的患者资格。结果和结论:c反应蛋白(CRP)、白细胞(WBC)、#中性、#淋巴和中性粒细胞与淋巴细胞比率(NLR)等参数的单一分析并不是一个很好的指标,不能作为辅助患者进行T2Dx检测的额外工具。区分T2Dx阳性和阴性患者的最敏感参数是IL-6和PCT的测量。适当的T2Dx检测资格可以显著减少启动靶向抗生素治疗的时间,并可能影响降低死亡率和改善长期治疗结果。
期刊介绍:
Journal of Clinical Laboratory Analysis publishes original articles on newly developing modes of technology and laboratory assays, with emphasis on their application in current and future clinical laboratory testing. This includes reports from the following fields: immunochemistry and toxicology, hematology and hematopathology, immunopathology, molecular diagnostics, microbiology, genetic testing, immunohematology, and clinical chemistry.