Immunomodulatory properties of a methanolic extract of Ficus lyrata mitigate inflammation in Complete Freund's Adjuvant-induced arthritis.

IF 4.6 2区医学 Q2 IMMUNOLOGY

Inflammopharmacology Pub Date : 2025-03-26 DOI:10.1007/s10787-025-01700-6

Anam Iftikhar, Mohammad Saleem, Syed Atif Raza, Adrian Achuthan, Rodney Luwor, Taha Muhammad, Umair Majeed

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引用次数: 0

Abstract

Ficus lyrata, renowned for its traditional use in alleviating rheumatic pain and inflammation, was validated for its purported anti-arthritic and antiinflammatory properties using InvivoandInvitromodels. In the in-vivo studies, carrageenan-induced acute oedema and a chronic arthritis induced by complete Freund's adjuvant (CFA) were employed. Oral dosing of methanolic extract from Ficus lyrata (m-FL) was administered at (250,500 and 750 mg/kg) as well as methotrexate at 1 mg/kg, significantly (p < 0.0001) demonstrated a dose dependent decrease in percent oedema/inflammation and notably reduced arthritis development in the CFA model, indicating strong anti-inflammatory effects over time. Further analysis revealed m-FL extract inhibited protein denaturation across all evaluated concentrations(1600,800,400,200,100,50 µg/ml) suggesting potential mechanisms for their anti-inflammatory action. Phytochemical analysis identified flavonoids and phenolic compounds in the extract. Gene expression analysis of m-FL extract treatment group using qPCR showed a significant (p < 0.0001) downregulation of various inflammatory markers (IL1,COX2,IL1β, NFκB, TNF,STAT-3,IL6) with simultaneous upregulation in the expression of antiinflammatory markers (IL4,10). The m-FL extract targets TNF-mediated decrease in interleukins specifically IL-1, IL1β and IL-6. Histopathological assessments and radiographic evaluations conducted subsequently demonstrated a significant decline in joint inflammation, bone erosion, and pannus formation, along with improved bone integrity and reduced inflammation in the m-FL extract-treated groups (p < 0.0001). Although the m-FL extract exhibited relatively stronger antioxidant activity compared to ascorbic acid in-vitro, the primary efficacy lies in the potent immunomodulatory and anti-arthritic effects. The m-FL extract showed potential as an adjunct therapy for managing inflammatory conditions.

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来源期刊

Inflammopharmacology IMMUNOLOGYTOXICOLOGY-TOXICOLOGY

CiteScore

8.00

自引率

3.40%

发文量

200

期刊介绍： Inflammopharmacology is the official publication of the Gastrointestinal Section of the International Union of Basic and Clinical Pharmacology (IUPHAR) and the Hungarian Experimental and Clinical Pharmacology Society (HECPS). Inflammopharmacology publishes papers on all aspects of inflammation and its pharmacological control emphasizing comparisons of (a) different inflammatory states, and (b) the actions, therapeutic efficacy and safety of drugs employed in the treatment of inflammatory conditions. The comparative aspects of the types of inflammatory conditions include gastrointestinal disease (e.g. ulcerative colitis, Crohn''s disease), parasitic diseases, toxicological manifestations of the effects of drugs and environmental agents, arthritic conditions, and inflammatory effects of injury or aging on skeletal muscle. The journal has seven main interest areas: -Drug-Disease Interactions - Conditional Pharmacology - i.e. where the condition (disease or stress state) influences the therapeutic response and side (adverse) effects from anti-inflammatory drugs. Mechanisms of drug-disease and drug disease interactions and the role of different stress states -Rheumatology - particular emphasis on methods of measurement of clinical response effects of new agents, adverse effects from anti-rheumatic drugs -Gastroenterology - with particular emphasis on animal and human models, mechanisms of mucosal inflammation and ulceration and effects of novel and established anti-ulcer, anti-inflammatory agents, or antiparasitic agents -Neuro-Inflammation and Pain - model systems, pharmacology of new analgesic agents and mechanisms of neuro-inflammation and pain -Novel drugs, natural products and nutraceuticals - and their effects on inflammatory processes, especially where there are indications of novel modes action compared with conventional drugs e.g. NSAIDs -Muscle-immune interactions during inflammation [...]