Exploration of biophoton characteristics of fresh Isatis indigotica fort leaves under salt and drought stresses and the feasibility analysis for the quality prediction of Isatidis Folium.

IF 4.1 2区 生物学 Q1 PLANT SCIENCES
Frontiers in Plant Science Pub Date : 2025-03-12 eCollection Date: 2025-01-01 DOI:10.3389/fpls.2025.1523636
Zhiying Wang, Baorui Cao, Yueyue Ma, Weifan Xu, Jialei Fu, Zhongwen Zhang, Jinxin Du, Tingting Deng, Jingxiang Pang, Meina Yang, Jinxiang Han
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引用次数: 0

Abstract

Introduction: Isatidis Folium, derived from the dried leaves of Isatis indigotica Fort, has been used for centuries as a traditional Chinese herb with antibacterial and antiviral properties. However, both the cultivation conditions and the growth status of Isatis indigotica Fort have been negatively affected by climatic and environmental degradation, which has made it challenging to accurately assess the quality of Isatidis Folium. The current quality control system for Isatidis Folium lacks precision and comprehensive identification indices, and importantly, the cultivation process has not been integrated into this system.

Methods: In this study, we proposed a novel method to distinguish between different stress subtypes in Isatis indigotica Fort based on biophoton emission and attempted to explore the potential relationship between the biophoton characteristics of fresh Isatis indigotica Fort leaves and the quality of Isatidis Folium. The delayed luminescence (DL) and spontaneous photon emission (SPE) characteristics of fresh Isatis indigotica Fort leaves under different stress conditions were detected using a biophoton detection system. An attempt was made to differentiate samples subjected to various stress treatments using biophoton characteristic parameters. Additionally, the content of active ingredients was determined by ultra-high performance liquid chromatography, and the inhibitory activity against Escherichia coli and Staphylococcus aureus was evaluated to identify the quality of Isatidis Folium. Several physiological indicators of fresh Isatis indigotica Fort leaves, including the photosynthetic pigment content, relative electrical conductivity, and reactive oxygen species production rate were also determined.

Result: The differences in physiological indices, active ingredient content, and inhibitory activity indicated that the stress conditions significantly inhibited the growth status of Isatis indigotica Fort leaves and the herbal quality. Meanwhile, biophoton characteristic parameters were obtained that could accurately and efficiently distinguish fresh Isatis indigotica Fort leaves between different stress subtypes: initial intensity of DL and counts per second of SPE. Both characteristic parameters were highly correlated with the physiological indicators and quality of Isatidis Folium.

Discussion: This study has preliminarily demonstrated the feasibility of utilizing biophoton detection technology for the quality evaluation of Isatidis Folium during cultivation for the first time and provided an improved method for distinguishing samples of various qualities.

盐和干旱胁迫下蓝靛鲜叶生物光子特性的探索及蓝靛叶品质预测的可行性分析。
isatiis Folium是由Isatis indigotica Fort的干叶子提取的,作为一种具有抗菌和抗病毒特性的传统中草药已经被使用了几个世纪。然而,由于气候和环境的恶化,蓝靛的栽培条件和生长状况都受到了不利的影响,这给蓝靛品质的准确评价带来了挑战。现有的板蓝叶质量控制体系缺乏精确和全面的鉴定指标,重要的是没有将栽培过程纳入该体系。方法:本研究提出了一种基于光子辐射的蓝靛不同胁迫亚型鉴别方法,并试图探索蓝靛鲜叶的光子特性与蓝靛叶品质之间的潜在关系。利用生物光子检测系统,对不同胁迫条件下青靛鲜叶的延迟发光(DL)和自发光子发射(SPE)特性进行了检测。利用生物光子特征参数对不同应力处理下的样品进行了区分。采用超高效液相色谱法测定其有效成分含量,并评价其对大肠杆菌和金黄色葡萄球菌的抑制活性,以鉴定板蓝叶的质量。测定了青靛鲜叶的光合色素含量、相对电导率和活性氧生成速率等生理指标。结果:生理指标、有效成分含量和抑制活性的差异表明,胁迫条件显著抑制了板蓝叶的生长状况和药材品质。同时,获得了能准确、高效区分不同胁迫亚型蓝靛鲜叶的生物光子特征参数:DL初始强度和每秒SPE计数。这两个特征参数与板蓝叶的生理指标和品质高度相关。讨论:本研究首次初步论证了利用生物光子检测技术进行板蓝叶栽培过程质量评价的可行性,并为不同品质样品的鉴别提供了一种改进的方法。
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来源期刊
Frontiers in Plant Science
Frontiers in Plant Science PLANT SCIENCES-
CiteScore
7.30
自引率
14.30%
发文量
4844
审稿时长
14 weeks
期刊介绍: In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches. Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.
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