Functional analysis of the StERF79 gene in response to drought stress in potato (Solanum tuberosum L.).

IF 4.3 2区生物学 Q1 PLANT SCIENCES

BMC Plant Biology Pub Date : 2025-03-27 DOI:10.1186/s12870-025-06417-w

Jingjing Wei, Ning Zhang, Yurong Deng, Shengyan Liu, Liang Yang, Xiaofeng Wang, Ruiting Wen, Huaijun Si

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引用次数: 0

Abstract

Background: The AP2/ERF (APETALA 2/ethylene-responsive element binding factors) is a class of superfamily of plant-specific transcription factor that play an important regulatory role in many physiological and biochemical processes in plants.

Results: In this study, overexpression of the StERF79 gene increased drought tolerance in potato plants, whereas StERF79 RNA interference expression (RNAi) lines decreased drought tolerance in potato plants. In addition, the superoxide dismutase (SOD), peroxide dismutase (POD), and catalase (CAT) activities, as well as proline (Pro) content of StERF79 transgenic lines, showed significantly higher results than those of the wild type (WT) potato plants under natural drought stress conditions, while the malondialdehyde (MDA) content was lower. The StERF79 transcription factor can respond to drought stress by interacting with a DRE cis-acting element in the promoter region of the downstream target gene (StDHN-2), and activating its expression, the result was validated by using yeast one hybrid (Y1H), Dual-Luciferase and β-glucuronidase (GUS) staining assays both in vivo and in vitro. The StDHN-2 gene is a member of the dehydrin (DHN) subfamily of the potato plant late embryonic developmentally abundant (LEA) protein family. LEA, hydrophilic proteins found in plants, serve as cellular dewatering protectants to prevent desiccation during various stresses.

Conclusion: The results could provide novel knowledge into the functional analysis of the StERF79 gene in positive regulation of the StDHN-2 gene to drought response and its possible mechanisms in potato plants.

Clinical trial number: Not applicable.

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来源期刊

BMC Plant Biology 生物-植物科学

CiteScore

8.40

自引率

3.80%

发文量

539

审稿时长

3.8 months

期刊介绍： BMC Plant Biology is an open access, peer-reviewed journal that considers articles on all aspects of plant biology, including molecular, cellular, tissue, organ and whole organism research.