Functional analysis of the StERF79 gene in response to drought stress in potato (Solanum tuberosum L.).

IF 4.3 2区 生物学 Q1 PLANT SCIENCES
Jingjing Wei, Ning Zhang, Yurong Deng, Shengyan Liu, Liang Yang, Xiaofeng Wang, Ruiting Wen, Huaijun Si
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引用次数: 0

Abstract

Background: The AP2/ERF (APETALA 2/ethylene-responsive element binding factors) is a class of superfamily of plant-specific transcription factor that play an important regulatory role in many physiological and biochemical processes in plants.

Results: In this study, overexpression of the StERF79 gene increased drought tolerance in potato plants, whereas StERF79 RNA interference expression (RNAi) lines decreased drought tolerance in potato plants. In addition, the superoxide dismutase (SOD), peroxide dismutase (POD), and catalase (CAT) activities, as well as proline (Pro) content of StERF79 transgenic lines, showed significantly higher results than those of the wild type (WT) potato plants under natural drought stress conditions, while the malondialdehyde (MDA) content was lower. The StERF79 transcription factor can respond to drought stress by interacting with a DRE cis-acting element in the promoter region of the downstream target gene (StDHN-2), and activating its expression, the result was validated by using yeast one hybrid (Y1H), Dual-Luciferase and β-glucuronidase (GUS) staining assays both in vivo and in vitro. The StDHN-2 gene is a member of the dehydrin (DHN) subfamily of the potato plant late embryonic developmentally abundant (LEA) protein family. LEA, hydrophilic proteins found in plants, serve as cellular dewatering protectants to prevent desiccation during various stresses.

Conclusion: The results could provide novel knowledge into the functional analysis of the StERF79 gene in positive regulation of the StDHN-2 gene to drought response and its possible mechanisms in potato plants.

Clinical trial number: Not applicable.

马铃薯StERF79基因在干旱胁迫下的功能分析。
背景:AP2/ERF (APETALA 2/乙烯响应元件结合因子)是一类植物特异性转录因子超家族,在植物的许多生理生化过程中起重要的调控作用。结果:在本研究中,StERF79基因的过表达增加了马铃薯植株的耐旱性,而StERF79 RNA干扰表达(RNAi)系降低了马铃薯植株的耐旱性。此外,在自然干旱胁迫条件下,StERF79转基因品系的超氧化物歧化酶(SOD)、过氧化物歧化酶(POD)和过氧化氢酶(CAT)活性以及脯氨酸(Pro)含量显著高于野生型(WT),丙二醛(MDA)含量显著低于野生型(WT)。StERF79转录因子通过与下游靶基因(StDHN-2)启动子区域的DRE顺式作用元件相互作用,激活其表达来响应干旱胁迫,并通过酵母一杂交(Y1H)、双荧光素酶(Dual-Luciferase)和β-葡萄糖醛酸酶(GUS)在体内和体外染色实验验证了这一结果。StDHN-2基因是马铃薯植物胚胎发育丰度蛋白家族脱氢蛋白亚家族的成员。LEA是一种存在于植物中的亲水性蛋白,它可以作为细胞脱水保护剂,在各种胁迫条件下防止细胞脱水。结论:该研究结果为分析马铃薯StDHN-2基因正调控stf79基因对干旱响应的功能及其可能机制提供了新的思路。临床试验号:不适用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Plant Biology
BMC Plant Biology 生物-植物科学
CiteScore
8.40
自引率
3.80%
发文量
539
审稿时长
3.8 months
期刊介绍: BMC Plant Biology is an open access, peer-reviewed journal that considers articles on all aspects of plant biology, including molecular, cellular, tissue, organ and whole organism research.
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