Pressurized fluid extraction of bioactive compounds from peanut by-products to promote waste recovery and circular economy

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Analytical and Bioanalytical Chemistry Pub Date : 2025-03-27 DOI:10.1007/s00216-025-05839-7

Clara Schumann, Beatriz Martín-Gómez, Ana Jano, Ana M. Ares, José Bernal

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引用次数: 0

Abstract

This work is based on the development and optimization of a pressurized liquid extraction method to obtain extracts from peanut shells with the highest possible amount/number of bioactive compounds, mainly flavonoids, with senolytic activity and antioxidant capacity. To achieve optimal extraction conditions, a design of experiments approach was employed to perform a limited and relatively reduced number of experiments. The extracts were consecutively analyzed by methods adapted to the peanut shell matrix to determine antioxidant capacity, total flavonoids, and total phenolic compounds. Additionally, a high-performance liquid chromatography coupled with diode array detection method was developed and validated to quantify individual phenolic compounds, with confirmation provided by mass spectrometry. Moreover, amino acid profiling was performed using gas chromatography coupled with mass spectrometry. Finally, the optimized extraction conditions and analytical methods were applied to analyze six commercial peanut shell samples. The results indicate that the optimized pressurized liquid extraction method using ethanol effectively extracts substantial amounts of bioactive compounds, especially flavonoids, which have broad applications across different industries. This contributes to a strategic valorization approach that promotes a Circular Economy.

Graphical Abstract

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花生副产品中生物活性物质的加压萃取促进废物回收和循环经济。

本研究的基础是开发和优化一种从花生壳中提取生物活性化合物（主要是黄酮类化合物）的加压液体提取方法，以获得具有抗衰老活性和抗氧化能力的最高含量/数量的提取物。为了获得最佳提取条件，采用实验设计方法进行有限且相对较少的实验。采用适用于花生壳基质的方法对提取液进行连续分析，测定其抗氧化能力、总黄酮和总酚类化合物含量。此外，开发并验证了高效液相色谱耦合二极管阵列检测方法，以定量单个酚类化合物，并通过质谱法进行了确认。此外，采用气相色谱-质谱法进行氨基酸谱分析。最后，将优化后的提取条件和分析方法应用于6种商品花生壳样品的分析。结果表明，优化后的乙醇加压液体提取方法可有效提取出大量的生物活性成分，其中黄酮类化合物的含量最高，在不同行业具有广泛的应用前景。这有助于促进循环经济的战略增值方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical and Bioanalytical Chemistry 化学-分析化学

CiteScore

8.00

自引率

4.70%

发文量

638

审稿时长

2.1 months

期刊介绍： Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.