Guilherme José Schwarzt Sampaio, Rodrigo de Almeida Romagna, Reginaldo Bezerra dos Santos, Rita de Cássia Ribeiro Gonçalves, Edésia Martins Barros de Sousa, Gracielle Ferreira Andrade, Rodrigo Rezende Kitagawa
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引用次数: 0
Abstract
The difficulty in treating cancer has led to several studies on the development of systems that perform targeted drug delivery, with the aim of increasing the effectiveness of treatment and reducing adverse effects. In this study, a series of chalcones were tested for cytotoxic action on gastric adenocarcinoma cells (AGS) and breast cancer cells (MCF-7) using the MTT-tetrazolium method, and significant cytotoxicity was demonstrated for 3-hydroxychalcone (CHO). The synthesis of mesoporous silica nanoparticles (MSNs) and their surface modification with 3-aminopropyltriethoxysilane (APTES) were carried out, and 3-hydroxychalcone was then incorporated into these nanomaterials. Mesoporous silica nanoparticles were characterized by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), elemental analysis (CHN), scanning electron microscopy (SEM), transmission electron microscopy (TEM), zeta potential, and nitrogen adsorption. In addition, in vitro release tests were carried out to verify the release profile of 3-hydroxychalcone from mesoporous silica samples. The results obtained showed that the mesoporous silica nanoparticles exhibited a gradual and prolonged release profile. In the cytotoxicity test with silica samples incorporated with 3-hydroxychalcone, significant cytotoxic activity was observed against AGS and MCF-7 cells, with the MSN-CHO sample exhibiting a better cytotoxic effect (IC50 of 12.93 to 22.30 μM) than 3-hydroxychalcone (IC50 of 47.58 to 47.97 μM). The results showed that the nanoparticles positively influenced the interaction of 3-hydroxychalcone with tumor cells. This is therefore an unprecedented study on the incorporation of 3-hydroxychalcone into mesoporous silica nanoparticles and its promising results in terms of cytotoxic activity against breast and gastric cancer cells.
期刊介绍：
The objective of the Journal of Nanoparticle Research is to disseminate knowledge of the physical, chemical and biological phenomena and processes in structures that have at least one lengthscale ranging from molecular to approximately 100 nm (or submicron in some situations), and exhibit improved and novel properties that are a direct result of their small size.
Nanoparticle research is a key component of nanoscience, nanoengineering and nanotechnology.
The focus of the Journal is on the specific concepts, properties, phenomena, and processes related to particles, tubes, layers, macromolecules, clusters and other finite structures of the nanoscale size range. Synthesis, assembly, transport, reactivity, and stability of such structures are considered. Development of in-situ and ex-situ instrumentation for characterization of nanoparticles and their interfaces should be based on new principles for probing properties and phenomena not well understood at the nanometer scale. Modeling and simulation may include atom-based quantum mechanics; molecular dynamics; single-particle, multi-body and continuum based models; fractals; other methods suitable for modeling particle synthesis, assembling and interaction processes. Realization and application of systems, structures and devices with novel functions obtained via precursor nanoparticles is emphasized. Approaches may include gas-, liquid-, solid-, and vacuum-based processes, size reduction, chemical- and bio-self assembly. Contributions include utilization of nanoparticle systems for enhancing a phenomenon or process and particle assembling into hierarchical structures, as well as formulation and the administration of drugs. Synergistic approaches originating from different disciplines and technologies, and interaction between the research providers and users in this field, are encouraged.
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