Localization and function of APC15 during mouse oocyte meiotic progression

IF 2.9 4区生物学 Q3 CELL BIOLOGY

Journal of Molecular Histology Pub Date : 2025-03-28 DOI:10.1007/s10735-025-10404-8

Shi-Bin Chao, Ren-Ren Zhang, Qing-Yuan Sun

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引用次数: 0

Abstract

The Anaphase-Promoting Complex/Cyclosome (APC/C) is a critical regulator of cell cycle progression, with APC15 serving as an essential subunit. While the role of APC15 in mitosis is well characterized, its function during meiosis remains poorly understood. In this study, we investigated the expression, subcellular localization, and potential role of APC15 during mouse oocyte meiotic progression. Using immunofluorescence and confocal microscopy, we observed dynamic changes in APC15 localization throughout meiotic progression. Knockdown of APC15 via siRNA did not affect spindle organization, but led to meiotic arrest at metaphase I (MI) and impaired the removal of BUB3 from kinetochores, suggesting a disruption in Spindle Assembly Checkpoint (SAC) inactivation. Our results highlight the involvement of APC15 in the regulation of SAC and the transition from metaphase to anaphase in oocytes. These findings contribute to our understanding of APC15’s role in meiotic regulation and provide insights into its potential impact on maintaining chromosomal stability during oocyte maturation.

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APC15在小鼠卵母细胞减数分裂过程中的定位和功能

后期促进复合物/环小体（APC/C）是细胞周期进程的关键调节因子，其中APC15是一个重要的亚基。虽然APC15在有丝分裂中的作用已被很好地描述，但其在减数分裂中的功能仍知之甚少。在这项研究中，我们研究了APC15在小鼠卵母细胞减数分裂过程中的表达、亚细胞定位和潜在作用。利用免疫荧光和共聚焦显微镜，我们观察了APC15定位在减数分裂过程中的动态变化。通过siRNA敲低APC15不会影响纺锤体组织，但会导致中期I （MI）减数分裂停滞，并损害着丝点上BUB3的去除，这表明纺锤体组装检查点（SAC）失活中断。我们的研究结果强调了APC15参与卵母细胞SAC的调控以及从中期到后期的转变。这些发现有助于我们理解APC15在减数分裂调控中的作用，并为其在卵母细胞成熟过程中维持染色体稳定性的潜在影响提供了见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Molecular Histology 生物-细胞生物学

CiteScore

5.90

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.