Evaluation of the antibacterial activity and mechanism of cannabigerol against drug-resistant Streptococcus iniae in vitro

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Archives of Microbiology Pub Date : 2025-03-27 DOI:10.1007/s00203-025-04311-5

Jianing Wu, Minmin Wang, Tingke He, Xiaonan Li, Bowen Liu, Fen Chen, Yi Geng, Weiming Lai, Xiaoli Huang, Defang Chen, Ping Ouyang

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Abstract

This study aimed to investigate the antibacterial effects and mechanism of cannabigerol against drug-resistant Streptococcus iniae. The determination of antibacterial activity was based on the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), growth curve analysis, time-kill assay, biofilm inhibition and eradication assessments. The antibacterial mechanism was explored by DNA leakage assay, assessment of cell membrane permeability, evaluation of cell membrane integrity, measurement of membrane potential, determination of respiratory chain dehydrogenase activity, and examination by using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The results demonstrated that cannabigerol effectively inhibited the growth and biofilm formation of Streptococcus iniae in vitro. Mechanistically, cannabigerol induced DNA leakage, impaired cell membrane integrity, hyperpolarized membrane potential, and reduced respiratory chain dehydrogenase activity in S. iniae. In conclusion, these findings suggest that cannabigerol inhibited the growth of S. iniae by disrupting the cell membrane.

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大麻酚对耐药牛链球菌体外抗菌活性及作用机制的评价

本研究旨在探讨大麻酚对耐药犬链球菌的抑菌作用及其机制。采用最小抑菌浓度（MIC）和最小杀菌浓度（MBC）、生长曲线分析、时间测定、生物膜抑制和根除评价等方法测定其抑菌活性。采用DNA渗漏试验、细胞膜通透性评价、细胞膜完整性评价、膜电位测定、呼吸链脱氢酶活性测定、扫描电镜（SEM）和透射电镜（TEM）检测等方法探讨其抗菌作用机制。结果表明，大麻酚能有效抑制猪链球菌的体外生长和生物膜的形成。在机制上，大麻酚诱导猪链球菌DNA渗漏、细胞膜完整性受损、膜电位超极化和呼吸链脱氢酶活性降低。综上所述，这些发现表明大麻酚通过破坏细胞膜来抑制葡萄球菌的生长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.