Antifungal Natural Products from the Culture Medium of Trichoderma orarium 18F0041

IF 1.3 4区生物学 Q4 CHEMISTRY, MEDICINAL

Phytochemistry Letters Pub Date : 2025-03-28 DOI:10.1016/j.phytol.2025.102945

Hui-Tzu Ni , Jih-Jung Chen , Hsia-Wei Liu , Jyh-Yih Leu , Ming-Der Wu , Yu-Hui Wei , Min Tseng , Yuan-Hsiang Yu , Ming-Jen Cheng

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引用次数: 0

Abstract

Fractionation of an EtOAc-soluble partition of a solid fermentation of the endophytic fungus Trichoderma orarium 18F0041 resulted in the isolation of two new compounds, a γ-butyrolactone, 5-hydroxy-3-(methoxymethyl)-4-methylfuran-2(5H)-one (1), and a methylpentanoid derivative, 5-ethoxy-4-hydroxy-3-methyl-5-oxopentanoic acid (2). Additionally, two further metabolites were also isolated for the first time from a natural source, namely 2-hydroxypyridin-3-yl acetate (3) and (+)-rel-(4S,5S)-4,5-dihydroxytetrahydro-2H-pyran-2-one (4). All structures were elucidated on the basis of extensive analyses of spectroscopic data and comparison with literature data. Their antifungal activity was also evaluated. Our results showed that some of the constituents possessed mild antifungal activity against Fusarium sp. LC8, Neopestalotiopsis sp. BCRC 35002, and Colletotrichum gloeosporioides BCRC 35178. Previously, δ-valerolactone derivatives were rarely reported in the genus Trichoderma. The discovery of these compounds in the fermentation products of this new Trichoderma species is therefore highly significant. Natural products of Trichoderma warrant further investigation as potential antifungal agents for biocontrol applications.

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野生木霉18F0041培养基抗真菌天然产物的研究

对内生真菌木霉orarium 18F0041固体发酵的乙酸乙酯可溶部分进行分离，分离出两个新化合物：γ-丁内酯，5-羟基-3-（甲氧基甲基）-4-甲基呋喃-2(5H)-one(1)和甲基戊烷衍生物，5-乙氧基-4-羟基-3-甲基-5-氧戊酸(2)。此外，还首次从天然来源分离到两个代谢物。即2-羟基吡啶-3-乙酸酯(3)和(+)-rel-(4S,5S)-4,5-二羟基四氢- 2h -吡喃-2- 1(4)。所有结构都是在广泛分析光谱数据并与文献数据比较的基础上确定的。并对其抗真菌活性进行了评价。结果表明，部分成分对镰刀菌LC8、新estestalotiopsis sp. BCRC 35002和gloeosporioides BCRC 35178具有较强的抗真菌活性。在此之前，δ-缬内酯衍生物在木霉属中很少报道。因此，在这种新的木霉物种的发酵产物中发现这些化合物是非常重要的。木霉的天然产物作为潜在的抗真菌剂在生物防治中的应用值得进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phytochemistry Letters 生物-生化与分子生物学

CiteScore

3.00

自引率

11.80%

发文量

190

审稿时长

34 days

期刊介绍： Phytochemistry Letters invites rapid communications on all aspects of natural product research including: • Structural elucidation of natural products • Analytical evaluation of herbal medicines • Clinical efficacy, safety and pharmacovigilance of herbal medicines • Natural product biosynthesis • Natural product synthesis and chemical modification • Natural product metabolism • Chemical ecology • Biotechnology • Bioassay-guided isolation • Pharmacognosy • Pharmacology of natural products • Metabolomics • Ethnobotany and traditional usage • Genetics of natural products Manuscripts that detail the isolation of just one new compound are not substantial enough to be sent out of review and are out of scope. Furthermore, where pharmacology has been performed on one new compound to increase the amount of novel data, the pharmacology must be substantial and/or related to the medicinal use of the producing organism.