Norovirus molecular trends in H preschoolers Post-NPI easing

IF 4.7 3区医学 Q1 INFECTIOUS DISEASES

Journal of Infection and Public Health Pub Date : 2025-03-18 DOI:10.1016/j.jiph.2025.102762

Yuan Tian , Xiqiao Du , Hong Gao , Mingyue Yuan , Yingchen Wang , Lei Shang , Yuhui Pan , Tuo Dong , Zhe Zhang

{"title":"Norovirus molecular trends in H preschoolers Post-NPI easing","authors":"Yuan Tian , Xiqiao Du , Hong Gao , Mingyue Yuan , Yingchen Wang , Lei Shang , Yuhui Pan , Tuo Dong , Zhe Zhang","doi":"10.1016/j.jiph.2025.102762","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Noroviruses (NoV) are key pathogens causing acute gastroenteritis (AGE) in children, with a significant disease burden, especially in developing nations. This study aims to track changes in NoV genotype epidemiology before and after the lifting of COVID-19 non-pharmacological interventions (NPIs) in Harbin.</div></div><div><h3>Methods</h3><div>In this study, we investigated fecal samples collected by the Harbin Children's Hospital from attended AGE preschoolers between January 2022 and December 2023. The detection of norovirus was performed using RT-qPCR. Later, the norovirus-positive samples were typed by conventional RT-PCR and Sanger sequencing. Phylogenetic analysis, recombination breakpoint analysis, and mutation analysis were also performed.</div></div><div><h3>Results</h3><div>230 NoV-positive samples were detected in the two years, with a positive rate of 39.1 %, belonging to NoV GⅡ. According to the partial sequences of the RNA-dependent RNA polymerase (RdRp) and VP1 gene sequence analysis, seven different genotypes were detected, being GII.4[P16] (61.5 %) the predominant one. Furthermore, the mutation analysis indicated that Harbin-Nov-066–2023 exhibited a mutation from valine to isoleucine at positions 290aa and 386aa in the VP1 region, respectively, compared to Harbin-Nov-022–2022. Additionally, strains from the rare type GⅡ.8[P8] were detected in 2022.</div></div><div><h3>Conclusions</h3><div>These findings may contribute novel data to the genetic database of norovirus in Harbin. This could facilitate investigations into the genetic evolution of this virus, developing vaccines to target dominant genotypes and the molecular basis of norovirus genetics.</div></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"18 5","pages":"Article 102762"},"PeriodicalIF":4.7000,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Infection and Public Health","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S187603412500111X","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}

引用次数: 0

Abstract

Background

Noroviruses (NoV) are key pathogens causing acute gastroenteritis (AGE) in children, with a significant disease burden, especially in developing nations. This study aims to track changes in NoV genotype epidemiology before and after the lifting of COVID-19 non-pharmacological interventions (NPIs) in Harbin.

Methods

In this study, we investigated fecal samples collected by the Harbin Children's Hospital from attended AGE preschoolers between January 2022 and December 2023. The detection of norovirus was performed using RT-qPCR. Later, the norovirus-positive samples were typed by conventional RT-PCR and Sanger sequencing. Phylogenetic analysis, recombination breakpoint analysis, and mutation analysis were also performed.

Results

230 NoV-positive samples were detected in the two years, with a positive rate of 39.1 %, belonging to NoV GⅡ. According to the partial sequences of the RNA-dependent RNA polymerase (RdRp) and VP1 gene sequence analysis, seven different genotypes were detected, being GII.4[P16] (61.5 %) the predominant one. Furthermore, the mutation analysis indicated that Harbin-Nov-066–2023 exhibited a mutation from valine to isoleucine at positions 290aa and 386aa in the VP1 region, respectively, compared to Harbin-Nov-022–2022. Additionally, strains from the rare type GⅡ.8[P8] were detected in 2022.

Conclusions

These findings may contribute novel data to the genetic database of norovirus in Harbin. This could facilitate investigations into the genetic evolution of this virus, developing vaccines to target dominant genotypes and the molecular basis of norovirus genetics.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Infection and Public Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH -INFECTIOUS DISEASES

CiteScore

13.10

自引率

1.50%

发文量

203

审稿时长

96 days

期刊介绍： The Journal of Infection and Public Health, first official journal of the Saudi Arabian Ministry of National Guard Health Affairs, King Saud Bin Abdulaziz University for Health Sciences and the Saudi Association for Public Health, aims to be the foremost scientific, peer-reviewed journal encompassing infection prevention and control, microbiology, infectious diseases, public health and the application of healthcare epidemiology to the evaluation of health outcomes. The point of view of the journal is that infection and public health are closely intertwined and that advances in one area will have positive consequences on the other. The journal will be useful to all health professionals who are partners in the management of patients with communicable diseases, keeping them up to date. The journal is proud to have an international and diverse editorial board that will assist and facilitate the publication of articles that reflect a global view on infection control and public health, as well as emphasizing our focus on supporting the needs of public health practitioners. It is our aim to improve healthcare by reducing risk of infection and related adverse outcomes by critical review, selection, and dissemination of new and relevant information in the field of infection control, public health and infectious diseases in all healthcare settings and the community.