Norovirus molecular trends in H preschoolers Post-NPI easing

IF 4.7 3区医学 Q1 INFECTIOUS DISEASES

Journal of Infection and Public Health Pub Date : 2025-03-18 DOI:10.1016/j.jiph.2025.102762

Yuan Tian , Xiqiao Du , Hong Gao , Mingyue Yuan , Yingchen Wang , Lei Shang , Yuhui Pan , Tuo Dong , Zhe Zhang

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引用次数: 0

Abstract

Background

Noroviruses (NoV) are key pathogens causing acute gastroenteritis (AGE) in children, with a significant disease burden, especially in developing nations. This study aims to track changes in NoV genotype epidemiology before and after the lifting of COVID-19 non-pharmacological interventions (NPIs) in Harbin.

Methods

In this study, we investigated fecal samples collected by the Harbin Children's Hospital from attended AGE preschoolers between January 2022 and December 2023. The detection of norovirus was performed using RT-qPCR. Later, the norovirus-positive samples were typed by conventional RT-PCR and Sanger sequencing. Phylogenetic analysis, recombination breakpoint analysis, and mutation analysis were also performed.

Results

230 NoV-positive samples were detected in the two years, with a positive rate of 39.1 %, belonging to NoV GⅡ. According to the partial sequences of the RNA-dependent RNA polymerase (RdRp) and VP1 gene sequence analysis, seven different genotypes were detected, being GII.4[P16] (61.5 %) the predominant one. Furthermore, the mutation analysis indicated that Harbin-Nov-066–2023 exhibited a mutation from valine to isoleucine at positions 290aa and 386aa in the VP1 region, respectively, compared to Harbin-Nov-022–2022. Additionally, strains from the rare type GⅡ.8[P8] were detected in 2022.

Conclusions

These findings may contribute novel data to the genetic database of norovirus in Harbin. This could facilitate investigations into the genetic evolution of this virus, developing vaccines to target dominant genotypes and the molecular basis of norovirus genetics.

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npi缓解后H学龄前儿童诺如病毒分子趋势

诺如病毒（NoV）是引起儿童急性胃肠炎（AGE）的主要病原体，具有重大的疾病负担，特别是在发展中国家。本研究旨在追踪哈尔滨市解除COVID-19非药物干预措施前后NoV基因型流行病学的变化。方法在本研究中，我们调查了哈尔滨儿童医院于2022年1月至2023年12月期间收集的AGE学龄前儿童的粪便样本。采用RT-qPCR检测诺如病毒。随后，采用常规RT-PCR和Sanger测序对诺如病毒阳性样本进行分型。系统发育分析、重组断点分析和突变分析。结果2年共检出NoV阳性样本230份，阳性率为39.1 %，属于NoV GⅡ。根据RNA依赖性RNA聚合酶（RdRp）部分序列和VP1基因序列分析，检测到7种不同的基因型，以GII.4[P16]（61.5 %）为优势基因型。突变分析表明，与Harbin-Nov-022-2022相比，Harbin-Nov-066-2023分别在VP1区290aa和386aa位点发生了缬氨酸向异亮氨酸的突变。此外，罕见的G型菌株Ⅱ。2022年共检出8例[P8]。结论本研究结果可为哈尔滨市诺如病毒遗传数据库的建立提供新的数据。这可能有助于调查该病毒的遗传进化，开发针对诺如病毒主要基因型和分子基础的疫苗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Infection and Public Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH -INFECTIOUS DISEASES

CiteScore

13.10

自引率

1.50%

发文量

203

审稿时长

96 days

期刊介绍： The Journal of Infection and Public Health, first official journal of the Saudi Arabian Ministry of National Guard Health Affairs, King Saud Bin Abdulaziz University for Health Sciences and the Saudi Association for Public Health, aims to be the foremost scientific, peer-reviewed journal encompassing infection prevention and control, microbiology, infectious diseases, public health and the application of healthcare epidemiology to the evaluation of health outcomes. The point of view of the journal is that infection and public health are closely intertwined and that advances in one area will have positive consequences on the other. The journal will be useful to all health professionals who are partners in the management of patients with communicable diseases, keeping them up to date. The journal is proud to have an international and diverse editorial board that will assist and facilitate the publication of articles that reflect a global view on infection control and public health, as well as emphasizing our focus on supporting the needs of public health practitioners. It is our aim to improve healthcare by reducing risk of infection and related adverse outcomes by critical review, selection, and dissemination of new and relevant information in the field of infection control, public health and infectious diseases in all healthcare settings and the community.