Interaction between OsLEC1 and OsHDA710 positively regulates callus formation in rice

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-03-22 DOI:10.1016/j.plaphy.2025.109826

Wenzhuo Zeng , Jingjing Li , Dandan Li , Jinhan Lu , Yidan Pan , Haonan Wei , Yangfan Chen , Qingyao Shu , Hongwu Bian , Fu Guo

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引用次数: 0

Abstract

Transgenic and gene editing technologies often begin with callus induction through embryo in vitro culture in rice. Mutations in both OsLEC1 (the rice homolog of Leafy Cotyledon 1) and OsHDA710 (encoding a deacetylase) can severely impair callus induction in embryo culture. However, whether these genes are part of a common regulatory module remains unclear. This study demonstrates defective scutellum-derived callus induction in novel OsLEC1 knockout (Oslec1) lines, indicating its crucial role in this process. Immunoprecipitation-mass spectrometry assays revealed that OsHDA710 interacts with OsLEC1 in embryo-derived callus. Yeast two-hybrid and bimolecular fluorescence complementation assays confirmed that OsLEC1 interacts with OsHDA710 via its C-terminal domain. Comparative transcriptome analyses revealed a set of shared genes that were upregulated in scutellum-derived callus of both Oslec1 and Oshda710 mutants. The upregulated genes included the nuclear factor Y family transcription factors, as well as genes involved in hormone biosynthesis and signaling, starch and lipid synthesis and metabolism, and abiotic stress responses. Our study indicates that OsLEC1 and OsHDA710 collaborate to regulate callus induction in rice through a common pathway.

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.