Surface Engineering Magnetic Nanoparticles with Redox and Biological Properties

IF 3.7 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Langmuir Pub Date : 2025-03-27 DOI:10.1021/acs.langmuir.4c05147

Jing Liu, Ye Chen, Feixiong Chen

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Abstract

Magnetic nanoparticles (MNPs) are recognized as valuable tools for derived electrochemical biosensors and offer immense potential for the efficient diagnosis and detection of disease biomarkers. Herein, a new step-by-step approach for the development of multifunctional MNPs that exhibit both redox and biological properties is described. First, chemical cross-linking was employed to label these MNPs with redox dyes (ferrocene, anthraquinone, or methylene blue). Bovine serum albumin (BSA) was then applied as the terminal protective layer. Next, click chemistry was employed to engineer immunoglobulin G (IgG) onto the surface of these redox MNPs (IgG number of 35 ± 8 per MNP), providing multifunctionality. Before and after surface engineering, these MNPs exhibited high-quality size distributions, as characterized by differential centrifugal sedimentation (DCS). Square-wave voltammetry was used to reveal the presence of 21.8 ± 1.3 ferrocene molecules on each anti-CD63-based redox MNP, and the anti-CD63 antibodies still maintained their bioactivity toward the CD63 antigen. These multifunctional MNPs could be promising tools for advancing the development of MNP-assisted electrochemical biosensors and meeting the needs of single-nanoparticle electrochemistry.

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来源期刊

Langmuir 化学-材料科学：综合

CiteScore

6.50

自引率

10.30%

发文量

1464

审稿时长

2.1 months

期刊介绍： Langmuir is an interdisciplinary journal publishing articles in the following subject categories: Colloids: surfactants and self-assembly, dispersions, emulsions, foams Interfaces: adsorption, reactions, films, forces Biological Interfaces: biocolloids, biomolecular and biomimetic materials Materials: nano- and mesostructured materials, polymers, gels, liquid crystals Electrochemistry: interfacial charge transfer, charge transport, electrocatalysis, electrokinetic phenomena, bioelectrochemistry Devices and Applications: sensors, fluidics, patterning, catalysis, photonic crystals However, when high-impact, original work is submitted that does not fit within the above categories, decisions to accept or decline such papers will be based on one criteria: What Would Irving Do? Langmuir ranks #2 in citations out of 136 journals in the category of Physical Chemistry with 113,157 total citations. The journal received an Impact Factor of 4.384*. This journal is also indexed in the categories of Materials Science (ranked #1) and Multidisciplinary Chemistry (ranked #5).