Effect of pre-chlorination on bioelectricity production and stabilization of excess sludge by microbial fuel cell

IF 11.4 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Water Research Pub Date : 2025-03-27 DOI:10.1016/j.watres.2025.123564

Dongye He , Yazhi Nong , Yanxi He , Yin Luo , Chuanfu Li , Jixian Gao , Chenyuan Dang , Jie Fu

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Abstract

Microbial fuel cell (MFC) is a technology that can generate electricity while degrading excess sludge. However, the complex components, intricate biological structures, and inhibitory compounds in sludge limit the application of MFC. Therefore, this study utilized chlorination as a sludge pretreatment method to improve the comprehensive performance of MFC in sludge treatment. Results showed that pre-chlorination at a dose of 0.2 mg/L increased output voltage of MFC by 500 % from approximately 100 mV to around 600 mV, and power density by 15.60 % from 3.15 W/m³ to 3.64 W/m³, and simultaneously increased the degradation of sludge MLSS (mixed liquor suspended solids), MLVSS (mixed liquor volatile suspended solids), EPS (extracellular polymeric substances) polysaccharide and protein by 9.64 %, 47.07 %, 18.63 % and 16.26 %, respectively. Molecular composition analysis of EPS in sludge by three-dimensional excitation emission matrix fluorescence spectroscopy (3D-EEM), Fourier transform infrared spectroscopy (FTIR) and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) indicated pre-chlorination significantly promoted the molecular transformation in MFC. The microbiome analysis of anode biofilm in MFC by scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), metagenomics and metametabolomics revealed that pre-chlorination facilitated the development of biomass, enrichment of electricity-producing bacteria (EPB), enhancement of electricity-producing activity and metabolic activity. Moreover, the sludge EPS was the importance source for the microbial metabolites in MFC was validated by the joint analysis of FT-ICR-MS and metametabolomics.

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预氯化对微生物燃料电池生物发电及剩余污泥稳定的影响

微生物燃料电池（MFC）是一种既能发电又能降解多余污泥的技术。然而，污泥中复杂的成分、复杂的生物结构和抑制成分限制了MFC的应用。因此，本研究采用氯化作为污泥预处理方法，提高MFC处理污泥的综合性能。结果表明，0.2 mg/L的预氯化处理使MFC的输出电压从约100 mV提高到约600 mV，提高了500%，功率密度从3.15 W/m³提高到3.64 W/m³，提高了15.60%，同时污泥对混液悬浮固体（MLSS）、混液挥发性悬浮固体（MLVSS）、胞外聚合物（EPS）、多糖和蛋白质的降解率分别提高了9.64%、47.07%、18.63%和16.26%。三维激发发射矩阵荧光光谱（3D-EEM）、傅里叶变换红外光谱（FTIR）和傅里叶变换离子回旋共振质谱（FT-ICR-MS）对污泥中EPS的分子组成分析表明，预氯化处理显著促进了MFC中的分子转化。通过扫描电镜（SEM）、共聚焦激光扫描显微镜（CLSM）、宏基因组学和代谢组学对MFC阳极生物膜的微生物组学分析表明，预氯化有利于生物质的发展，有利于产电细菌（EPB）的富集，增强了产电活性和代谢活性。此外，FT-ICR-MS和代谢组学联合分析验证了污泥EPS是MFC中微生物代谢物的重要来源。

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来源期刊

Water Research 环境科学-工程：环境

CiteScore

20.80

自引率

9.40%

发文量

1307

审稿时长

38 days

期刊介绍： Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include: •Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management; •Urban hydrology including sewer systems, stormwater management, and green infrastructure; •Drinking water treatment and distribution; •Potable and non-potable water reuse; •Sanitation, public health, and risk assessment; •Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions; •Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment; •Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution; •Environmental restoration, linked to surface water, groundwater and groundwater remediation; •Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts; •Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle; •Socio-economic, policy, and regulations studies.