Immunohistochemical Evaluation of Bone Remodeling Following Compressive Force on Mandibular Condyle.

IF 3.6 3区 生物学 Q1 BIOLOGY
Ioannis Lyros, Ioannis A Tsolakis, Georgia Kotantoula, Konstantinos Tosios, Vilaras George, Nikolaos Nikitakis, Efstratios Ferdianakis, Theodoros Lykogeorgos, Eleni Fora, Apostolos I Tsolakis
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引用次数: 0

Abstract

Excessive mandibular growth is largely affected by genetic factors and its orthodontic management is challenging. The present study utilized histophotometry and aimed to investigate immunohistochemical alterations in rat condyles following posterior mandibular displacement using a fixed intraoral functional appliance. Seventy-two male Wistar rats were divided into two equal groups, experimental and control, each consisting of three equal subgroups. The animals were sacrificed after 30, 60, and 90 days, and their condyles were isolated and examined microscopically for potential immunohistochemical changes. Statistically significant differences in RANKL appeared at days 30 and 60, while noteworthy changes regarding TRAP were evidenced at days 30 and 90. In the experimental group, RANKL decreased statistically significantly between days 60 and 90, while in the control group, the RANKL measured significantly increased at day 90 compared to days 30 and 60 alike. In the experimental group, TRAP appeared significantly decreased at day 60 vs. 30, while at day 90 vs. 60, it was found to be significantly increased. In the control group, there were no significant changes in TRAP. Posterior mandibular displacement may cause immunohistochemical changes in the rat condylar bone, and such outcomes should be considered when planning similar procedures in humans.

免疫组织化学评价下颌髁受压后骨重塑。
下颌生长过快很大程度上受遗传因素影响,其正畸治疗具有挑战性。本研究采用组织光度法,目的是研究使用固定口内功能矫治器后下颌后移位大鼠髁突的免疫组织化学变化。将72只雄性Wistar大鼠分为实验组和对照组,每组分为三个相等的亚组。分别于30、60、90天后处死,分离髁突,显微镜下观察免疫组化变化。RANKL在第30天和第60天出现统计学差异,TRAP在第30天和第90天出现显著变化。实验组RANKL在第60 ~ 90天显著降低,对照组RANKL在第90天与第30、60天比较均显著升高。在实验组中,TRAP在第60天比第30天明显下降,而在第90天比第60天明显增加。在对照组中,TRAP无明显变化。下颌后移位可能会引起大鼠髁突骨的免疫组织化学变化,在计划人类类似手术时应考虑这些结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biology-Basel
Biology-Basel Biological Science-Biological Science
CiteScore
5.70
自引率
4.80%
发文量
1618
审稿时长
11 weeks
期刊介绍: Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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