Antarctic Soil and Viable Microbiota After Long-Term Storage at Constant -20 °C.

IF 3.6 3区 生物学 Q1 BIOLOGY
Cristian-Emilian Pop, Sergiu Fendrihan, Nicolai Crăciun, Garbis Vasilighean, Daniela Ecaterina Chifor, Florica Topârceanu, Andreea Florea, Dan Florin Mihăilescu, Maria Mernea
{"title":"Antarctic Soil and Viable Microbiota After Long-Term Storage at Constant -20 °C.","authors":"Cristian-Emilian Pop, Sergiu Fendrihan, Nicolai Crăciun, Garbis Vasilighean, Daniela Ecaterina Chifor, Florica Topârceanu, Andreea Florea, Dan Florin Mihăilescu, Maria Mernea","doi":"10.3390/biology14030222","DOIUrl":null,"url":null,"abstract":"<p><p>During an Antarctic expedition that took place in December 2010-January 2011 in the East Antarctic coastal region, soil samples were collected in aseptic conditions and stored for over a decade in freezers at -20 °C. Due to the shortly afterward passing of the Antarctic researcher in charge, Teodor Negoiță, the samples remained unintentionally frozen for a long period and were made available for research 13 years later. A chemical analysis of soil as well as screening for viable microbial presence was performed; soil analysis was conducted via inductively coupled plasma atomic emission spectroscopy (ICP-AES) and Fourier-transform infrared spectroscopy coupled with attenuated total reflection (FTIR-ATR). The presence of aerobic and facultative aerobic microbiotas was evaluated through a Biolog Ecoplates assay, and isolated strains were 16S sequenced for final taxonomic identification. The results obtained new insights into Antarctic soil characteristics from both chemical and microbiological aspects, even after over a decade of conservation.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 3","pages":""},"PeriodicalIF":3.6000,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11940283/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biology-Basel","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/biology14030222","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

During an Antarctic expedition that took place in December 2010-January 2011 in the East Antarctic coastal region, soil samples were collected in aseptic conditions and stored for over a decade in freezers at -20 °C. Due to the shortly afterward passing of the Antarctic researcher in charge, Teodor Negoiță, the samples remained unintentionally frozen for a long period and were made available for research 13 years later. A chemical analysis of soil as well as screening for viable microbial presence was performed; soil analysis was conducted via inductively coupled plasma atomic emission spectroscopy (ICP-AES) and Fourier-transform infrared spectroscopy coupled with attenuated total reflection (FTIR-ATR). The presence of aerobic and facultative aerobic microbiotas was evaluated through a Biolog Ecoplates assay, and isolated strains were 16S sequenced for final taxonomic identification. The results obtained new insights into Antarctic soil characteristics from both chemical and microbiological aspects, even after over a decade of conservation.

求助全文
约1分钟内获得全文 求助全文
来源期刊
Biology-Basel
Biology-Basel Biological Science-Biological Science
CiteScore
5.70
自引率
4.80%
发文量
1618
审稿时长
11 weeks
期刊介绍: Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信