Antarctic Soil and Viable Microbiota After Long-Term Storage at Constant -20 °C.

IF 3.6 3区 生物学 Q1 BIOLOGY
Cristian-Emilian Pop, Sergiu Fendrihan, Nicolai Crăciun, Garbis Vasilighean, Daniela Ecaterina Chifor, Florica Topârceanu, Andreea Florea, Dan Florin Mihăilescu, Maria Mernea
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引用次数: 0

Abstract

During an Antarctic expedition that took place in December 2010-January 2011 in the East Antarctic coastal region, soil samples were collected in aseptic conditions and stored for over a decade in freezers at -20 °C. Due to the shortly afterward passing of the Antarctic researcher in charge, Teodor Negoiță, the samples remained unintentionally frozen for a long period and were made available for research 13 years later. A chemical analysis of soil as well as screening for viable microbial presence was performed; soil analysis was conducted via inductively coupled plasma atomic emission spectroscopy (ICP-AES) and Fourier-transform infrared spectroscopy coupled with attenuated total reflection (FTIR-ATR). The presence of aerobic and facultative aerobic microbiotas was evaluated through a Biolog Ecoplates assay, and isolated strains were 16S sequenced for final taxonomic identification. The results obtained new insights into Antarctic soil characteristics from both chemical and microbiological aspects, even after over a decade of conservation.

在恒定-20°C长期储存后的南极土壤和活菌群。
在2010年12月至2011年1月在南极东部沿海地区进行的一次南极考察中,土壤样本在无菌条件下收集,并在-20°C的冰柜中储存了十多年。由于负责南极研究的特奥多尔Negoiță不久就去世了,这些样本在无意中被冷冻了很长一段时间,直到13年后才被用于研究。进行了土壤化学分析和活菌筛选;采用电感耦合等离子体原子发射光谱(ICP-AES)和衰减全反射傅里叶变换红外光谱(FTIR-ATR)对土壤进行了分析。通过Biolog Ecoplates测定好氧菌群和兼性好氧菌群的存在,并对分离菌株进行16S测序以进行最终的分类鉴定。即使经过十多年的保护,研究结果也从化学和微生物方面对南极土壤特征有了新的认识。
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来源期刊
Biology-Basel
Biology-Basel Biological Science-Biological Science
CiteScore
5.70
自引率
4.80%
发文量
1618
审稿时长
11 weeks
期刊介绍: Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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